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Zentrale Abteilung für Mikroskopie - Imaging Core Facility

Sebastian Markert

PhD Student
Zentrale Abteilung für Mikroskopie
Am Hubland
97074Würzburg
Germany
Gebäude: Biozentrum

Sebastian Markert is a neurobiologist interested in presynaptic ultrastructures, the function of their proteins, and the correlation between synaptic architecture and activity. He obtained his Bachelor’s degree at the Julius-Maximilians University of Würzburg in the department of bioinformatics and then moved on to cell and developmental biology, where he did his Master thesis on trypanosome genetics and development. Since 2013 he is working as PhD-student in the group of Christian Stigloher.

Sebastian Markert is mainly using the motor neurons of the roundworm Caenorhabditis elegans as a model for presynaptic architecture. He focuses in particular on the more subtle aspects of the synaptic build-up, the research of which requires excellent tissue preservation and imaging resolution. With channelrhodopsins, light-activatable ion-channels, it is possible to selectively activate neurons in a tightly controlled manner. By combining such optogenetic approaches with high pressure freezing for close-to-native preservation of ultrastructures and EM tomography for nanoscale resolution imaging, deeper insights into synaptic architecture are finally feasible.
In his PhD thesis Sebastian Markert is correlating synaptic activity states (manipulated via optogenetics) with presynaptic ultrastructure. By looking at several select neurological mutants he also tries to identify the proteins involved in the build-up of certain structural features in the presynapse and thus aims for the deduction of their functions.

2017

  • Markert, S.M., Bauer, V., Muenz, T.S., Jones, N.G., Helmprobst, F., Britz, S., Sauer, M., Rössler, W., Engstler, M., Stigloher, C.(2017)“Chapter 2 - 3D subcellular localization with superresolution array tomography on ultrathin sections of various species”, in Müller-Reichert, T. and Verkade, P., eds., Correlative Light And Electron Microscopy Iii, Methods In Cell Biology, Academic Press, 21 - 47, available: http://www.sciencedirect.com/science/article/pii/S0091679X17300481.
     

2016

  • Markert, S.M., Britz, S., Proppert, S., Lang, M., Witvliet, D., Mulcahy, B., Sauer, M., Zhen, M., Bessereau, J.-L., Stigloher, C.(2016)“Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome”, Neurophotonics, 3(4), 041802, available: https://doi.org/10.1117%2F1.nph.3.4.041802.
     

2018

  • Link, J., Paouneskou, D., Velkova, M., Daryabeigi, A., Laos, T., Labella, S., Barroso, C., Piñol, S.P., Montoya, A., Kramer, H., Woglar, A., Baudrimont, A., Markert, S.M., Stigloher, C., Martinez-Perez, E., Dammermann, A., Alsheimer, M., Zetka, M., Jantsch, V.(2018)“Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase”, Developmental Cell, 45(2), 212 - 225.e7, available: http://www.sciencedirect.com/science/article/pii/S1534580718302387.
     
  • Kaltdorf, K.V., Theiss, M., Markert, S.M., Zhen, M., Dandekar, T., Stigloher, C., Kollmannsberger, P.(2018)“Automated classification of synaptic vesicles in electron tomograms of C. elegans using machine learning”, PLOS ONE, 13(10), 1-22, available: https://doi.org/10.1371/journal.pone.0205348.
     

2017

  • Schieber, N.L., Machado, P., Markert, S.M., Stigloher, C., Schwab, Y., Steyer, A.M.(2017)“Chapter 4 - Minimal resin embedding of multicellular specimens for targeted FIB-SEM imaging”, in Müller-Reichert, T. and Verkade, P., eds., Correlative Light And Electron Microscopy Iii, Methods In Cell Biology, Academic Press, 69 - 83, available: http://www.sciencedirect.com/science/article/pii/S0091679X17300493.
     
  • Markert, S.M., Bauer, V., Muenz, T.S., Jones, N.G., Helmprobst, F., Britz, S., Sauer, M., Rössler, W., Engstler, M., Stigloher, C.(2017)“Chapter 2 - 3D subcellular localization with superresolution array tomography on ultrathin sections of various species”, in Müller-Reichert, T. and Verkade, P., eds., Correlative Light And Electron Microscopy Iii, Methods In Cell Biology, Academic Press, 21 - 47, available: http://www.sciencedirect.com/science/article/pii/S0091679X17300481.
     
  • García-Fernández, E., Koch, G., Wagner, R.M., Fekete, A., Stengel, S.T., Schneider, J., Mielich-Süss, B., Geibel, S., Markert, S.M., Stigloher, C., Lopez, D.(2017)“Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance”, Cell, 171(6), 1354 - 1367.e20, available: http://www.sciencedirect.com/science/article/pii/S0092867417311996.
     

2016

  • Jahn, M.T., Markert, S.M., Ryu, T., Ravasi, T., Stigloher, C., Hentschel, U., Moitinho-Silva, L.(2016)“Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and transcriptional profiling”, Scientific Reports, 6, 35860--, available: https://doi.org/10.1038/srep35860.
     
  • Markert, S.M., Britz, S., Proppert, S., Lang, M., Witvliet, D., Mulcahy, B., Sauer, M., Zhen, M., Bessereau, J.-L., Stigloher, C.(2016)“Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome”, Neurophotonics, 3(4), 041802, available: https://doi.org/10.1117%2F1.nph.3.4.041802.
     

2014

  • Batram, C., Jones, N.G., Janzen, C.J., Markert, S.M., Engstler, M.(2014)“Expression site attenuation mechanistically links antigenic variation and development in Trypanosoma brucei, eLife, 20;3:e02324, available: https://doi.org/10.7554/eLife.02324.
     

2012

  • Markert, S.M., Müller, T., Koetschan, C., Friedl, T., Wolf, M.(2012)“‘Y’Scenedesmus (Chlorophyta, Chlorophyceae): the internal transcribed spacer 2 rRNA secondary structure re-revisited”, Plant Biology, 14(6), 987-996, available: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1438-8677.2012.00576.x.