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    Chair of Biochemistry

    Lab & Teaching

    Courses & Course  Materials

    Aktuelle Methoden der Proteinchromatografie

    (5. Semester, Nr. 0732145)

    Kursbeschreibung mit Bildern

     Download der Kursanleitung (.pdf, German)

    Reinigung rekombinanter Histidase aus Escherichia coli

    (im Molekularbiologischen Praktikum, Nr. 0732142)

    Versuchsbeschreibung

     Download der Versuchsanleitung (.pdf, German)

     Reinigungstabelle als Word-Datei

     Tabellen für Aktivitäts- und Proteinbestimmungen als Word-Datei

       

    Basic Pymol course using ADH coordinates

    Download .pdf (German)

    Recipes

    Alternative Polymeric Precipitants Screen 

    Alternative, water-soluble polymeric precipitants are a valuable alternative to the popular polyethylene glycols (PEGs). In our lab, some difficult proteins could only be crystallized with particular, otherwise rarely used polymers. We have developped a screen  based on such precipitants - it is commercially available from Molecular Dimensions Ltd under the brand name MIDAS.

    Download .xls (the original Version as described in Grimm et al., Acta Cryst. D 66, 685-697)

    Download .pdf (the slightly modified version sold by MDL) 

    A rich defined medium for efficient SeMet labelling in difficult cases

    Download .pdf