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    Chair of Bioinformatics

    Program details 2020

    Online Lectures and labs will be held daily from 9.00 a.m. to 5.15 p.m..

    Sunday September 20th

    9:00 a.m. Registration (compulsory): Zoom-Meeting with Urlike Rapp-Galmiche

    Course Certificates

    Dear Course Members,
    here are the details on getting your course certificate:

    standard certificate (3 ECTS) you simply send a short summary on the course days regarding the scientific contents and what the key  messages to learn were for every day (a total of 1-2 pages is enough; but this is no course evaluation but a scientific summary of the course contents) to Dr. Rapp-Galmiche, she will then send out the course certificate subsequently.

    For a 5 ECTS certificate you send a summary of a particular topic (you can pick any lecture topic you like, please contact the lecturer of choice per e-mail and cc Dr. Rapp-Galmiche so that she knows from whom to expect the grading of the essay). Here the key point is to give a summary on the topic (about 4 pages, looking at current literature and giving proper references to current literature and of course just remember what has been taught in the course) and then write 3-4 pages about your own opinion and investigation on the topic. You may confirm the view presented in the lecture but you are also welcome to extend it by own suggestions or also point out the critical points, what is not yet clear, where more experiments etc. are warranted. Aim here to finish during December to send in the first draft. After corrections from the supervisor, the deadline for the revision is 9th of February. The summaries will be re-evaluated for completeness and content. After this the certificate for the course is sent to you.For this more demanding essay on a topic of the summer school in infection biology you can also get the course certificate with 5 ECTS and a grading (international A-E scale).

    Monday September 21st

    Systems Biology of Infection

    Lecturer: Prof. Dr. Thomas Dandekar (Homepage)

    Chair, Bioinformatics

    Pseudomonas Syringae attacks the host -- how do you understand the host response?
    (A) Phenotype: Pst (104 CFU/ml) infection; red arrows indicate pathogen inoculation; black arrow indicates mock (10mM MgCl2) inoculation. Symptoms weare photographed three days post pathogen inoculation (DPPI). (B) Network: Topology of Pst- mediated hormone disease networks in Arabidopsis. Connectivity among nodes is based either on activation (->) or inhibition (-|). Node designation: blue, enzymes of hormone biosynthesis and degradation; yellow, active hormone molecules; green, host regulatory factors; red, Pst- originated pathogenicity factors responsible for triggering immunity in Arabidopsis; pink, “PR-1”, marker node for immunity against the infection of Pst in Arabidopsis. All nodes are denoted by abbreviations.
    More information on these questions is found in our plant cell paper: Naseem M, Philippi N, Hussain A, Wangorsch G, Ahmed N, Dandekar T. Integrated systems view on networking by hormones in Arabidopsis immunity reveals multiple crosstalk for cytokinin. Plant Cell. 2012 May;24(5):1793-814.
    However, the same techniques can also be applied (and are shown in the course) to study human infection defense against pathogens. 

    Schedule

    Location: ONLINE MEETING

    Equipment: computers – with camera

    09:00-10:00 Introduction: Prof. Thomas Dandekar, Department of Bioinformatics University of Würzburg (chair) and EMBL Heidelberg
    Title: "Systems biology of infection – bioinformatical approaches"

    Including administration: attendance, insurance

    10:00-11:30 Part I Database and Interactomics

    • Dr. Chunguang Liang, Department of Bioinformatics University of Würzburg
      Title: "Databases in infection biology"

    11:30-14:15 Part II Getting into -omics: transcriptomics and proteomics

    12:30-13:30 Lunch break

    • 13:30-14:00 Dr. Elena Bencúrová,  Laboratory of Biomedicine, Microbiology and Immunology,
      University of Veterinary Medicine and Pharmacy in Kosice, Slovakia and University of Würzburg (lecture theatre A102)
      Title: "Proteomics in infection biology"
    • 14:00-14:15 Discussion

    14:15-15:00 Break

    15:00-16:30 Part III Current trends in systems biology

    • Prof. Alicia Ponte-Sucre, Humboldt professor, Insituto de Medicina Experimental, Universidad Central de Venezuela, Caracas
      Title 1: "Host-parasite interaction in Leishmania, neuropeptides and chemotaxis"
      Title 2: "Technology and ethics in research, an appropriate world for children"

    16:30-18:00 Part IV Drug design and signalling in infection biology

    • Dr. Meik Kunz, Chair of Medical Informatics, Friedrich-Alexander-University of Erlangen-Nürnberg
      Title: "Drug target identification in infection biology"
    • Prof. Thomas Dandekar, Department of Bioinformatics University of Würzburg (chair) and EMBL Heidelberg
      Title: "Current trends of modelling in infection biology"

    Useful link:

    http://www.nature.com/news/mapping-identifies-best-targets-for-malaria-prevention-1.10781

    References:
    Systems Biology
    A Textbook
    Klipp, Edda / Liebermeister, Wolfram / Wierling, Christoph / Kowald, Axel
    https://www.wiley-vch.de/de/fachgebiete/naturwissenschaften/systems-biology-978-3-527-33636-4

    Arthur Lesk "introduction to bioinformatics"
    https://www.amazon.de/Introduction-Bioinformatics-Arthur-Lesk/dp/0199651566

    For German speaking students the book by Andrea Hansen is fine
    https://www.springer.com/de/book/9783764362539

    as well as my own (with Meik Kunz)  
    https://www.springer.com/de/book/9783662546970?gclid=EAIaIQobChMIsMG0n4ba5AIVzeR3Ch1zaQDqEAQYASABEgL1__D_BwE

     


    Tuesday September 22nd

    Intracellular S. aureus

    Lecturer: Dr. Martin Fraunholz (Homepage)

    Figure: Staphylococcus aureus (cyan) in phagolysosomes of human cells (yellow)

    Topic area: Microscopic analysis of intracellular bacterial host-pathogen interactions: Practical image analysis with Fiji/ImageJ (basics & motion tracking, feature counting)

    Upon infection Staphylococcus aureus is readily phagocytosed by macrophages and neutrophils but is also to invade epithelial and endothelial cells, fibroblast, osteoblasts, and keratinocytes. The uptake by latter so-called non-professional phagocytes has been proposed to play a role in evasion of the innate immune system. Further survival of phagolysosomal killing by S. aureus may also lead to disseminating infections within migrating phagocytes. We and others have shown that S. aureus is capable of escaping from host cell phagosomes. One of our methods is microscopy based and relies on the recruitment of a cytoplasmically expressed host cell marker that is recruited to the bacterial cell wall upon phagosomal membrane rupture.

    We will address theoretical and practical aspects of confocal laser scanning microscopy as well as the underlying fluorophores. We will analyze images of uninfected and infected human cells using Fiji/ImageJ, a tool which is handy for other image analysis aspects, too.

    Participants should bring a USB Thumb drive, if they want to keep their own personalized Fiji/ImageJ version.

    Schedule

    Location: ONLINE MEETING

    Equipment: computers – with webcam

    09:00-10:00 Introduction: Martin Fraunholz, Chair of Microbiology, University of Würzburg


    10:00-12:30 Fiji/ImageJ to analyze biological data

    • „Installation“ and Basics of biological image acquisition

    12:30-13:30 Lunch break

    13:30-16:00 Fiji/ImageJ to analyze biological data

    • Image/Movie analysis in multiple dimension, Image quantification

    Wednesday September 23rd

    Location: ONLINE MEETING

    09:00-09:30     Visiting Germany: discussion round with Juan Prada Salcedo
    09:30-10:30     Round table
                               discussion possibilities for young researchers, possibilities infection biology
                               (Prof. Thomas Dandekar)
    11:00                 Research on Trypanosomes and Leishmania: opportunity for questions and discussion (Dr. Susanne Kramer)


    Thursday September 24th

    Trypanosomes and Leishmania

    Location: ONLINE MEETING

    Lecturer: Dr. Susanne Kramer (Homepage)

    Zoology I

    SLOT 1: 9:00-10:30 a.m.

    9:00-10:00   Trypanosoma brucei: introduction to the parasite and disease
                          Lecture (Susanne Kramer)

    10:00-10:30 The Cure (Sleeping Sickness)
                          Movie (Medicin Sans Frontiers)

    ZOOM-Coffee: 10:30-11:00 a.m.

                          Bring your own coffee and meet the spakers and each other
                          Social Meeting (students and lecturers)

    SLOT 2: 11:00-12:30 a.m.

    11:00-11:30 DNDT (German network against Neglected Tropical Diseases)
                          Lecture with discussion (Markus Engstler)

    11:30-12:00 Tsetse Flies
                          - Breeding in the laboratory
                             Movie (Lidia Schegelski and Susanne Kramer)
                          - Dissection and experiments
                            Insights from the Bench (Stephan Löwe and Jaime Lisack)

    12:00-12:30 More than parasites: Trypanosomes as model systems
                          Lecture with discussion (Susanne Kramer)

    LUNCH BREAK: 12:30-13:45 p.m.

    SLOT 3: 13:45-14:30 p.m.

    13:45-14:15 Trypanosoma cruzi: introduction to the parasite and disease
                          Lecture with discussion (Claudia Moreira)

    14:15-14:30 Working with T. cruzi in the lab, Master student report
                          Insights from the Bench (Bernardo Gabiatti)

    ZOOM-Coffee: 14:30-15:00 p.m.

                          Bring your own coffee and meet the spakers and each other
                          Social Meeting (students and lecturers)

    SLOT 4: 15:00-17:00 p.m.

    15:00-15:45 "Surveillance and prevention of Chagas in Latino America, how different countries have deal with the disease" (Alicia Ponte-Sucre)
                          Lecture with discussion (Alicia Ponte-Sucre)

    15:45-16:30 "Leishmania concepts and challenges, diagnosis, chemotherapy and drug resistance"
                           Lecture (Alicia Ponte-Sucre)

    16:30-16:45 Working with Leishmania in the lab, PhD student report
                          Insights from The Bench (Elisa Rauh)

    16:45-17:00 Conclusions, final discussion
                          Discussion (all)

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    Friday September 25th

    Parasitic Helminths

    Lecturer: Prof. Klaus Brehm (Homepage)

    Medical Parasitology

    Institute of Hygiene and Microbiology

    Figure: Echinokokkus

     

    Parasitic Flatworms: A tale of mortality and immortality.

    Parasitic helminths are a major cause of so-called ‘Neglected Diseases’. And Neglected Diseases are called ‘neglected’ because nobody (or only very few) has/have an interest to study them. So, why are we doing it? This is something we’re going to figure out in the ‘Parasitic Helminth’-day of the summer school. We shall learn that the development of novel therapies is not the only motivation to do infectious disease research and that these parasites (like many others) are highly fascinating from the view-point of immunologists as well as cell-, developmental- and evolutionary biologists. Above all, we shall learn how molecular research can be done in a ‘non-mainstream’ setting and which strategies should be followed to establish an infectious disease model system from scratch.

    Schedule

    09:00-12:00 Self-preparation on the topic (using materials provided in WueCampus)

    13:00-15:30 Lecture and video (Prof. Klaus Brehm)

    15:30-16:00 Conclusions, final discussion (all)