Confocal Laser Scanning Microscope (CLSM) with spectral detector

The Nikon C1 is a standard confocal microscope with 3 separate single channel detectors (PMTs). Additionally the emission light can be spectrally resolved by switching to a spectral detector. Using 32 PMTs the spectral resolution can be varied between 2.5, 5 and 10nm. Resolving the spectrum of the emission enables to correct for scattering of tissue, autofluorescence or split overlapping emission spectra of dyes.

Microscope-body: Nikon TiE
Objective: 100x Oil, NA 1.49, Apo-TIRF
100x Siliconoil, NA 1.3
Laser: 405nm, 488nm, 561nm, 639nm
Detection:3 PMTs
Spectral Detector, 32 Channels, spectral resolution 2.5/5/10nm

Spectral separation of SiR700-Tubulin (Magenta) and SiR-Actin (Cyan) using 640nm excitation

Emissionspectra of SiR700-Tubulin (Magenta) and SiR-Actin (Cyan)