LOCUS       pE-35S-HA-   7045 bp    DNA   circular  SYN       27-JAN-2005
DEFINITION  binary vector constructed from pEXSG and pENSG-vectors.
ACCESSION   pEXSG CFP
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|342611602|
COMMENT     VNTDBDATE|364580748|
COMMENT     VNTNAME|pE-35S-HA-GW|
COMMENT     VNTAUTHORNAME|Caroline Mayer|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTUDF|E.coli-Stamm|1|E416|
COMMENT     VNTUDF|freezer position|1|P421|
FEATURES             Location/Qualifiers
     misc_feature    complement (76..360)
                     /gene="pAnos"
                     /vntifkey="21"
                     /label=Misc_Feature(pAnos)_1
     CDS             complement (367..918)
                     /gene="pat"
                     /vntifkey="4"
                     /label=CDS(pat)_1
     misc_feature    complement (940..1250)
                     /gene="Pnos"
                     /vntifkey="21"
                     /label=Misc_Feature(Pnos)_2
     misc_feature    1260..1958
                     /gene="P35SS"
                     /vntifkey="21"
                     /label=Misc_Feature(P35SS)_3
     misc_feature    2010..2133
                     /gene="attR1"
                     /vntifkey="21"
                     /label=attR1
     CDS             2243..2902
                     /gene="CmR"
                     /vntifkey="4"
                     /label=CDS(CmR)_2
     misc_feature    3793..4009
                     /gene="'pA35S"
                     /vntifkey="21"
                     /label=Misc_Feature('pA35S)_6
     CDS             complement(4080..4105)
                     /gene="RB"
                     /product="right border"
                     /vntifkey="4"
                     /label=CDS(RB)_5
     misc_feature    4220..4928
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
                     /vntifkey="21"
                     /label=Misc_Feature(RK2\ori)_7
     CDS             5091..5951
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin resistance A.tumefaciens"
                     /vntifkey="4"
                     /label=CDS(bla)_6
     misc_feature    6020..6712
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
                     /vntifkey="21"
                     /label=Misc_Feature(ColE1\ori)_8
     CDS             7021..1
                     /gene="LB"
                     /product="left border"
                     /vntifkey="4"
                     /label=CDS(LB)_7
     misc_feature    3590..3714
                     /vntifkey="21"
                     /label=attR2
     CDS             3244..3546
                     /vntifkey="4"
                     /label=ccdB
BASE COUNT     1796 a      1696 c      1790 g      1763 t 
ORIGIN
        1 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatga tcgaatacta 
       61 acgtctctac cagatatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg 
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat 
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta 
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca 
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag 
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc 
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat 
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc 
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta 
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc 
      661 cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc 
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt 
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg 
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg 
      901 gcgtcgttct gggctcatgg tagatccccc tcgatcgagt tgagagtgaa tatgagactc 
      961 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt 
     1021 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg 
     1081 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc 
     1141 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact 
     1201 cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcggtttg ggcgcgcccg 
     1261 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc 
     1321 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat 
     1381 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac 
     1441 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt 
     1501 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct 
     1561 cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta ttgagacttt 
     1621 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt 
     1681 catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg 
     1741 aaaggctatc gttcaagaat gcctctaccg acagtggtcc caaagatgga cccccaccca 
     1801 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat 
     1861 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt 
     1921 cctctatata aggaagttca tttcatttgg agaggacctc gaggtcgacg gtatcgataa 
     1981 gcttgatatc gaattcctgc agcccatcaa caagtttgta caaaaaagct gaacgagaaa 
     2041 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat 
     2101 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt 
     2161 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt 
     2221 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat 
     2281 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta 
     2341 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca 
     2401 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt 
     2461 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac 
     2521 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt 
     2581 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta 
     2641 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt 
     2701 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat 
     2761 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca 
     2821 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga 
     2881 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta 
     2941 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag 
     3001 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc 
     3061 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc 
     3121 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg 
     3181 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt 
     3241 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat 
     3301 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca 
     3361 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa 
     3421 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa 
     3481 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg 
     3541 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg 
     3601 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat 
     3661 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatgg 
     3721 gggttatcca tacgatgttc cagattatgc tgtcggcgcc ggttggtctc atcctcaatt 
     3781 tgaaaaataa gtctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct 
     3841 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata 
     3901 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa 
     3961 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgaccg ggcggccgcc 
     4021 accgcggtgg agggggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt 
     4081 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 
     4141 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgtaca tcaccgacga 
     4201 gcaaggcaag accgagcgcc tttccgacgc tcaccgggct ggttgccctc gccgctgggc 
     4261 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac 
     4321 accggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg 
     4381 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag 
     4441 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga 
     4501 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat 
     4561 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg 
     4621 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga 
     4681 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa 
     4741 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg 
     4801 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc 
     4861 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat 
     4921 ggcagcgctg gcagtccttg ccattgccgg gatcggggca gtaacgggat gggcgatcag 
     4981 cccgacaagc tacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     5041 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 
     5101 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 
     5161 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 
     5221 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 
     5281 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 
     5341 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 
     5401 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 
     5461 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 
     5521 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 
     5581 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 
     5641 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 
     5701 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 
     5761 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 
     5821 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 
     5881 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 
     5941 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 
     6001 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     6061 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     6121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     6181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     6241 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 
     6301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     6361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     6421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     6481 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     6541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     6601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     6661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     6721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gactctagct 
     6781 agaggatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag 
     6841 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca 
     6901 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc 
     6961 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg 
     7021 gtggcaggat atattgtggt gtaaa 
//
