LOCUS       pE-SPYCE-G   7315 bp    DNA   circular            31-AUG-2007
DEFINITION  Split-YFP vector constructed from pE.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|342626358|
COMMENT     VNTDBDATE|456147975|
COMMENT     VNTNAME|pE-SPYCE-GW|
COMMENT     VNTAUTHORNAME|Caroline Mayer|
COMMENT     VNTUDF|E.coli-Stamm|1|E422|
COMMENT     VNTUDF|freezer position|1|P423|
FEATURES             Location/Qualifiers
     misc_feature    7..36
                     /vntifkey="21"
                     /label=HA-tag
     misc_feature    37..288
                     /vntifkey="21"
                     /label=C-terminal\YFP
     misc_feature    complement(5433..5717)
                     /gene="pAnos"
                     /vntifkey="21"
                     /label=Misc_Feature(pAnos)_1
     CDS             complement(5724..6275)
                     /gene="pat"
                     /vntifkey="4"
                     /label=CDS(pat)_1
     misc_feature    complement(6297..6607)
                     /gene="Pnos"
                     /vntifkey="21"
                     /label=Misc_Feature(Pnos)_2
     misc_feature    6617..7315
                     /gene="P35SS"
                     /vntifkey="21"
                     /label=Misc_Feature(P35SS)_3
     misc_feature    322..445
                     /gene="attR1"
                     /vntifkey="21"
                     /label=attR1
     CDS             555..1214
                     /gene="CmR"
                     /vntifkey="4"
                     /label=CDS(CmR)_2
     misc_feature    2105..2321
                     /gene="'pA35S"
                     /vntifkey="21"
                     /label=Misc_Feature('pA35S)_6
     CDS             complement(2392..2417)
                     /gene="RB"
                     /product="right border"
                     /vntifkey="4"
                     /label=CDS(RB)_5
     misc_feature    2532..3240
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
                     /vntifkey="21"
                     /label=Misc_Feature(RK2\ori)_7
     CDS             3403..4263
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin resistance A.tumefaciens"
                     /vntifkey="4"
                     /label=CDS(bla)_6
     misc_feature    4332..5024
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
                     /vntifkey="21"
                     /label=Misc_Feature(ColE1\ori)_8
     CDS             5333..5358
                     /gene="LB"
                     /product="left border"
                     /vntifkey="4"
                     /label=CDS(LB)_7
     misc_feature    1902..2026
                     /vntifkey="21"
                     /label=attR2
     CDS             1556..1858
                     /vntifkey="4"
                     /label=ccdB
     primer_bind     270..289
                     /vntifkey="28"
                     /label=Primer\SPYCE
BASE COUNT     1866 a      1789 c      1859 g      1801 t 
ORIGIN
        1 tcgaggatgt acccatacga tgttccagat tacgctgaca agcagaagaa cggcatcaag 
       61 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 
      121 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 
      181 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 
      241 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggt aagcttgata 
      301 tcgaattcct gcagcccatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat 
      361 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 
      421 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt 
      481 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag 
      541 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 
      601 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 
      661 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 
      721 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 
      781 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 
      841 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 
      901 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 
      961 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 
     1021 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 
     1081 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct 
     1141 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 
     1201 agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt 
     1261 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 
     1321 aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 
     1381 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 
     1441 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 
     1501 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca 
     1561 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 
     1621 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 
     1681 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 
     1741 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 
     1801 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 
     1861 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 
     1921 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 
     1981 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat gggggttatc 
     2041 catacgatgt tccagattat gctgtcggcg ccggttggtc tcatcctcaa tttgaaaaat 
     2101 aagtctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt 
     2161 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg 
     2221 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta 
     2281 tcaataaaat ttctaattcc taaaaccaaa atccagtgac cgggcggccg ccaccgcggt 
     2341 ggagggggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat 
     2401 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag 
     2461 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgta catcaccgac gagcaaggca 
     2521 agaccgagcg cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc 
     2581 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccggccg 
     2641 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt 
     2701 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt 
     2761 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc 
     2821 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt 
     2881 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc 
     2941 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag 
     3001 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt 
     3061 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg 
     3121 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc 
     3181 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc 
     3241 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgacaa 
     3301 gctaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     3361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
     3421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     3481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     3541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     3601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     3661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     3721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     3781 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     3841 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     3901 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
     3961 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     4021 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     4081 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
     4141 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     4201 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     4261 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
     4321 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
     4381 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     4441 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     4501 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     4561 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 
     4621 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     4681 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     4741 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     4801 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     4861 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     4921 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     4981 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     5041 tttttacggt tcctggcctt ttgctggcct tttgctcaca tggactctag ctagaggatc 
     5101 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg 
     5161 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa 
     5221 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta 
     5281 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg 
     5341 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt 
     5401 ttaatgatcg aatactaacg tctctaccag atatcagctt gcatgccggt cgatctagta 
     5461 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta 
     5521 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg 
     5581 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa 
     5641 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac 
     5701 gatctgcttg actctagggg tcatcagatt tcggtgacgg gcaggaccgg acggggcggc 
     5761 accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag 
     5821 ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc 
     5881 gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac 
     5941 ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg gggggagacg 
     6001 tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg gcccgcgtag 
     6061 gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga 
     6121 cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg 
     6181 cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca 
     6241 cggcggatgt cggccgggcg tcgttctggg ctcatggtag atccccctcg atcgagttga 
     6301 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat 
     6361 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata 
     6421 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc 
     6481 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg 
     6541 ggggtcataa cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt 
     6601 cggtttgggc gcgcccggtc tcagaagacc agagggctat tgagactttt caacaaaggg 
     6661 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 
     6721 cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg 
     6781 ttcaagaatg cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc 
     6841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg 
     6901 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag 
     6961 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 
     7021 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc 
     7081 catcattgcg ataaaggaaa ggctatcgtt caagaatgcc tctaccgaca gtggtcccaa 
     7141 agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc 
     7201 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta 
     7261 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacc 
//
