LOCUS       pE-SPYCE(-   5734 bp    DNA   circular            22-FEB-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|366886889|
COMMENT     VNTDBDATE|366886890|
COMMENT     VNTNAME|pE-SPYCE(-)|
COMMENT     VNTAUTHORNAME|Caroline Mayer|
FEATURES             Location/Qualifiers
     misc_feature    5428..5457
                     /vntifkey="21"
                     /label=HA-tag
     misc_feature    5458..5709
                     /vntifkey="21"
                     /label=C-terminal\YFP
     misc_feature    complement(3539..3823)
                     /gene="pAnos"
                     /vntifkey="21"
                     /label=Misc_Feature(pAnos)_1
     CDS             complement(3830..4381)
                     /gene="pat"
                     /vntifkey="4"
                     /label=CDS(pat)_1
     misc_feature    complement(4403..4713)
                     /gene="Pnos"
                     /vntifkey="21"
                     /label=Misc_Feature(Pnos)_2
     misc_feature    4723..5421
                     /gene="P35SS"
                     /vntifkey="21"
                     /label=Misc_Feature(P35SS)_3
     misc_feature    211..427
                     /gene="'pA35S"
                     /vntifkey="21"
                     /label=Misc_Feature('pA35S)_6
     CDS             complement(498..523)
                     /gene="RB"
                     /product="right border"
                     /vntifkey="4"
                     /label=CDS(RB)_5
     misc_feature    638..1346
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
                     /vntifkey="21"
                     /label=Misc_Feature(RK2\ori)_7
     CDS             1509..2369
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin resistance A.tumefaciens"
                     /vntifkey="4"
                     /label=CDS(bla)_6
     misc_feature    2438..3130
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
                     /vntifkey="21"
                     /label=Misc_Feature(ColE1\ori)_8
     CDS             3439..3464
                     /gene="LB"
                     /product="left border"
                     /vntifkey="4"
                     /label=CDS(LB)_7
     misc_feature    8..132
                     /vntifkey="21"
                     /label=attR2
     primer_bind     5691..5710
                     /vntifkey="28"
                     /label=Primer\SPYCE
BASE COUNT     1416 a      1454 c      1479 g      1385 t 
ORIGIN
        1 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg 
       61 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt 
      121 gtacaaagtg gttgatgggg gttatccata cgatgttcca gattatgctg tcggcgccgg 
      181 ttggtctcat cctcaatttg aaaaataagt ctagagtccg caaaaatcac cagtctctct 
      241 ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag 
      301 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat 
      361 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc 
      421 agtgaccggg cggccgccac cgcggtggag ggggatcaga ttgtcgtttc ccgccttcag 
      481 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt 
      541 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 
      601 tgtgtacatc accgacgagc aaggcaagac cgagcgcctt tccgacgctc accgggctgg 
      661 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt 
      721 cgaagccgtg tgcgagacac cggccgccgg cgttgtggat acctcgcgga aaacttggcc 
      781 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg 
      841 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa 
      901 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg 
      961 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga 
     1021 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag 
     1081 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac 
     1141 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag 
     1201 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc 
     1261 ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa 
     1321 cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga tcggggcagt 
     1381 aacgggatgg gcgatcagcc cgacaagcta cccctatttg tttatttttc taaatacatt 
     1441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     1501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     1561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     1621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     1681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     1741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     1801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     1861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     1921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     1981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     2041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     2101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     2161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     2221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     2281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     2341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     2401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     2461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     2521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     2641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     2701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     2761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     2821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     2881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     2941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     3001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     3061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     3121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     3181 ctcacatgga ctctagctag aggatcacag gcagcaacgc tctgtcatcg ttacaatcaa 
     3241 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 
     3301 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 
     3361 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 
     3421 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac 
     3481 aacttaataa cacattgcgg acgtttttaa tgatcgaata ctaacgtctc taccagatat 
     3541 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta 
     3601 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat 
     3661 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac 
     3721 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa 
     3781 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg 
     3841 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca 
     3901 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 
     3961 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 
     4021 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 
     4081 ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 
     4141 tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 
     4201 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 
     4261 gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 
     4321 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca 
     4381 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg 
     4441 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac 
     4501 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac 
     4561 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta 
     4621 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc 
     4681 atgatcagat tgtcgtttcc cgccttcggt ttgggcgcgc ccggtctcag aagaccagag 
     4741 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc 
     4801 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca 
     4861 tcattgcgat aaaggaaagg ctatcgttca agaatgcctc taccgacagt ggtcccaaag 
     4921 atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 
     4981 agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctcgtctac tccaagaata 
     5041 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat 
     5101 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag 
     5161 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag 
     5221 aatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa 
     5281 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac 
     5341 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 
     5401 tcatttcatt tggagaggac ctcgaggatg tacccatacg atgttccaga ttacgctgac 
     5461 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     5521 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     5581 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc 
     5641 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     5701 ctgtacaagg taagcttgat atcgaattcc tgca 
//
