LOCUS       pE-SPYNE-G   7531 bp    DNA   circular            27-JAN-2005
DEFINITION  Split-YFP vector constructed from pE.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|342626013|
COMMENT     VNTDBDATE|364580795|
COMMENT     VNTNAME|pE-SPYNE-GW|
COMMENT     VNTAUTHORNAME|Caroline Mayer|
COMMENT     VNTUDF|freezer position|1|P422|
COMMENT     VNTUDF|E.coli-Stamm|1|E421|
FEATURES             Location/Qualifiers
     misc_feature    7..39
                     /vntifkey="21"
                     /label=myc-tag
     misc_feature    40..504
                     /vntifkey="21"
                     /label=N-terminal\YFP
     misc_feature    complement(5649..5933)
                     /gene="pAnos"
                     /vntifkey="21"
                     /label=Misc_Feature(pAnos)_1
     CDS             complement(5940..6491)
                     /gene="pat"
                     /vntifkey="4"
                     /label=CDS(pat)_1
     misc_feature    complement(6513..6823)
                     /gene="Pnos"
                     /vntifkey="21"
                     /label=Misc_Feature(Pnos)_2
     misc_feature    6833..7531
                     /gene="P35SS"
                     /vntifkey="21"
                     /label=Misc_Feature(P35SS)_3
     misc_feature    538..661
                     /gene="attR1"
                     /vntifkey="21"
                     /label=attR1
     CDS             771..1430
                     /gene="CmR"
                     /vntifkey="4"
                     /label=CDS(CmR)_2
     misc_feature    2321..2537
                     /gene="'pA35S"
                     /vntifkey="21"
                     /label=Misc_Feature('pA35S)_6
     CDS             complement(2608..2633)
                     /gene="RB"
                     /product="right border"
                     /vntifkey="4"
                     /label=CDS(RB)_5
     misc_feature    2748..3456
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
                     /vntifkey="21"
                     /label=Misc_Feature(RK2\ori)_7
     CDS             3619..4479
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin resistance A.tumefaciens"
                     /vntifkey="4"
                     /label=CDS(bla)_6
     misc_feature    4548..5240
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
                     /vntifkey="21"
                     /label=Misc_Feature(ColE1\ori)_8
     CDS             5549..5574
                     /gene="LB"
                     /product="left border"
                     /vntifkey="4"
                     /label=CDS(LB)_7
     misc_feature    2118..2242
                     /vntifkey="21"
                     /label=attR2
     CDS             1772..2074
                     /vntifkey="4"
                     /label=ccdB
     primer_bind     473..492
                     /vntifkey="28"
                     /label=Primer\SPYNE
BASE COUNT     1908 a      1848 c      1934 g      1841 t 
ORIGIN
        1 tcgaggatgg agcaaaagtt gatttctgag gaggatctta tggtgagcaa gggcgaggag 
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac 
      241 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 
      481 agccacaacg tctatatcat ggccggaagc ttgatatcga attcctgcag cccatcaaca 
      541 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat 
      601 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta 
      661 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt 
      721 ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa 
      781 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 
      841 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 
      901 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 
      961 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 
     1021 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 
     1081 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 
     1141 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 
     1201 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 
     1261 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 
     1321 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa 
     1381 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat 
     1441 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa 
     1501 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg 
     1561 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 
     1621 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 
     1681 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 
     1741 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag 
     1801 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 
     1861 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 
     1921 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 
     1981 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 
     2041 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag 
     2101 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 
     2161 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 
     2221 cagctttctt gtacaaagtg gttgatgggg gttatccata cgatgttcca gattatgctg 
     2281 tcggcgccgg ttggtctcat cctcaatttg aaaaataagt ctagagtccg caaaaatcac 
     2341 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt 
     2401 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa 
     2461 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa 
     2521 accaaaatcc agtgaccggg cggccgccac cgcggtggag ggggatcaga ttgtcgtttc 
     2581 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga 
     2641 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg 
     2701 tccatttgta tgtgtacatc accgacgagc aaggcaagac cgagcgcctt tccgacgctc 
     2761 accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca 
     2821 gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg cgttgtggat acctcgcgga 
     2881 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc 
     2941 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc 
     3001 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac 
     3061 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg 
     3121 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt 
     3181 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt 
     3241 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt 
     3301 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc 
     3361 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct 
     3421 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga 
     3481 tcggggcagt aacgggatgg gcgatcagcc cgacaagcta cccctatttg tttatttttc 
     3541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 
     3601 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 
     3661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 
     3721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     3781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     3841 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     3901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     3961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     4021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     4081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     4141 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     4201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     4261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     4321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     4381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     4441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     4501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     4561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     4621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     4681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     4741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 
     4801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     4861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     4921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     4981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     5041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     5101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     5161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     5221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     5281 tggccttttg ctcacatgga ctctagctag aggatcacag gcagcaacgc tctgtcatcg 
     5341 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt 
     5401 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc 
     5461 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc 
     5521 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg 
     5581 acgcttagac aacttaataa cacattgcgg acgtttttaa tgatcgaata ctaacgtctc 
     5641 taccagatat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata 
     5701 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg 
     5761 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta 
     5821 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat 
     5881 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat 
     5941 cagatttcgg tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc 
     6001 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg 
     6061 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca 
     6121 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg 
     6181 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag 
     6241 tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc 
     6301 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc 
     6361 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg 
     6421 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt 
     6481 tctgggctca tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg 
     6541 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc 
     6601 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag 
     6661 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt 
     6721 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa 
     6781 ttctccgctc atgatcagat tgtcgtttcc cgccttcggt ttgggcgcgc ccggtctcag 
     6841 aagaccagag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat 
     6901 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct 
     6961 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agaatgcctc taccgacagt 
     7021 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc 
     7081 acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctcgtctac 
     7141 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa 
     7201 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa 
     7261 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct 
     7321 atcgttcaag aatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa 
     7381 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat 
     7441 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat 
     7501 ataaggaagt tcatttcatt tggagaggac c 
//
