LOCUS       pE-SPYNE(-   7101 bp    DNA   circular            22-FEB-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|366887105|
COMMENT     VNTDBDATE|366887105|
COMMENT     VNTNAME|pE-SPYNE(-)|
COMMENT     VNTAUTHORNAME|Caroline Mayer|
FEATURES             Location/Qualifiers
     misc_feature    5253..5285
                     /vntifkey="21"
                     /label=myc-tag
     misc_feature    5286..5750
                     /vntifkey="21"
                     /label=N-terminal\YFP
     misc_feature    complement(3364..3648)
                     /gene="pAnos"
                     /vntifkey="21"
                     /label=Misc_Feature(pAnos)_1
     CDS             complement(3655..4206)
                     /gene="pat"
                     /vntifkey="4"
                     /label=CDS(pat)_1
     misc_feature    complement(4228..4538)
                     /gene="Pnos"
                     /vntifkey="21"
                     /label=Misc_Feature(Pnos)_2
     misc_feature    4548..5246
                     /gene="P35SS"
                     /vntifkey="21"
                     /label=Misc_Feature(P35SS)_3
     misc_feature    5784..5907
                     /gene="attR1"
                     /vntifkey="21"
                     /label=attR1
     CDS             6017..6676
                     /gene="CmR"
                     /vntifkey="4"
                     /label=CDS(CmR)_2
     misc_feature    36..252
                     /gene="'pA35S"
                     /vntifkey="21"
                     /label=Misc_Feature('pA35S)_6
     CDS             complement(323..348)
                     /gene="RB"
                     /product="right border"
                     /vntifkey="4"
                     /label=CDS(RB)_5
     misc_feature    463..1171
                     /gene="RK2 ori"
                     /product="ori for A.tumefaciens"
                     /vntifkey="21"
                     /label=Misc_Feature(RK2\ori)_7
     CDS             1334..2194
                     /gene="bla"
                     /product="Ampicillin resistance E.coli / Carbenicillin resistance A.tumefaciens"
                     /vntifkey="4"
                     /label=CDS(bla)_6
     misc_feature    2263..2955
                     /gene="ColE1 ori"
                     /product="ori for E.coli"
                     /vntifkey="21"
                     /label=Misc_Feature(ColE1\ori)_8
     CDS             3264..3289
                     /gene="LB"
                     /product="left border"
                     /vntifkey="4"
                     /label=CDS(LB)_7
     primer_bind     5719..5738
                     /vntifkey="28"
                     /label=Primer\SPYNE
BASE COUNT     1805 a      1758 c      1827 g      1711 t 
ORIGIN
        1 gccggttggt ctcatcctca atttgaaaaa taagtctaga gtccgcaaaa atcaccagtc 
       61 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag 
      121 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt 
      181 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 
      241 aatccagtga ccgggcggcc gccaccgcgg tggaggggga tcagattgtc gtttcccgcc 
      301 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga 
      361 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat 
      421 ttgtatgtgt acatcaccga cgagcaaggc aagaccgagc gcctttccga cgctcaccgg 
      481 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac 
      541 gccgtcgaag ccgtgtgcga gacaccggcc gccggcgttg tggatacctc gcggaaaact 
      601 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg 
      661 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct 
      721 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc 
      781 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg 
      841 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat 
      901 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg 
      961 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta 
     1021 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc 
     1081 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc 
     1141 gcgaacggcc tcaccccaaa aatggcagcg ctggcagtcc ttgccattgc cgggatcggg 
     1201 gcagtaacgg gatgggcgat cagcccgaca agctacccct atttgtttat ttttctaaat 
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     1321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     1381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     1441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     1501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     1561 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     1621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     1681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     1741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     1801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     1861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     1921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     1981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     2041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     2101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     2161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     2221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     2281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     2341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     2401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     2461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 
     2521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     2581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     2641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     2701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     2761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     2821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     2881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     2941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     3001 ttttgctcac atggactcta gctagaggat cacaggcagc aacgctctgt catcgttaca 
     3061 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt 
     3121 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct 
     3181 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg 
     3241 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct 
     3301 tagacaactt aataacacat tgcggacgtt tttaatgatc gaatactaac gtctctacca 
     3361 gatatcagct tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta 
     3421 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact 
     3481 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc 
     3541 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat 
     3601 cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg gtcatcagat 
     3661 ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca gctgccagaa 
     3721 acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc 
     3781 atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac 
     3841 cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc 
     3901 cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta 
     3961 ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc 
     4021 ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg 
     4081 ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt 
     4141 gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg 
     4201 gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta attggatacc 
     4261 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata 
     4321 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat 
     4381 taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa 
     4441 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc 
     4501 cgctcatgat cagattgtcg tttcccgcct tcggtttggg cgcgcccggt ctcagaagac 
     4561 cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 
     4621 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa 
     4681 tgccatcatt gcgataaagg aaaggctatc gttcaagaat gcctctaccg acagtggtcc 
     4741 caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc 
     4801 ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacactctcg tctactccaa 
     4861 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt 
     4921 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 
     4981 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt 
     5041 tcaagaatgc ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg 
     5101 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca 
     5161 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 
     5221 gaagttcatt tcatttggag aggacctcga ggatggagca aaagttgatt tctgaggagg 
     5281 atcttatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 
     5341 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 
     5401 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 
     5461 ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca 
     5521 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 
     5581 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 
     5641 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 
     5701 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc ggaagcttga 
     5761 tatcgaattc ctgcagccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa 
     5821 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact 
     5881 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact 
     5941 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg 
     6001 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca 
     6061 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca 
     6121 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt 
     6181 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat 
     6241 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt 
     6301 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca 
     6361 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc 
     6421 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag 
     6481 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa 
     6541 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt 
     6601 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg 
     6661 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta 
     6721 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc 
     6781 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag 
     6841 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg 
     6901 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg 
     6961 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg 
     7021 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag 
     7081 agtgatatta ttgacacgcc c 
//
