Lecture 3: Results

1. unknown amino acid sequence
- the sequence is coding for a protein originated from Galdieria sulphuraria
- the supposed protein function is a cellulose-binding protein or glycine cleavage system protein R
- yellow highlighted are sequences scoring below threshold on previous iteration
- the protein in the UniProt database: https://www.uniprot.org/uniprot/J3QW32

2. UniProt
- BRCA2_Human is more related to BRCA1 from mice than to BRCA1 from human
- additional information about annotations and amino acid properties are also provided

3. SMART
- following domains were found for BRCA1_Human: RING domain, low complexity region, coiled coil and BRCT domain
- the protein shows 84 phosporylations

4. Histon-acetyltransferase
- Prosite: you will find 2 motifs, a Gcn5-related N-acetyltransferase (GNAT) and a Bromodomain
- AnDom: the first hit will also be a N-acetly transferase

5. Glutathione reductase
- you get several hits regarding to the glutathione reductase
- PFAM: two matches (Pyridine nucleotide-disulphide oxidoreductase and Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain)
- InterPro: 7 matches. The most important matches due to the protein function of glutathione reductase are
	- FAD binding domain
	- NAD binding domain
	- catalytic domain (glutathione reductase, pyridine nucleotide-disulphide oxireductase
	- dimerisation domain