Lecture 6: Results


1. Signal Peptide database
- the Swiss-Prot Annoteted Keywords are Secreted; Signal; Stuctural protein
- belongs to the actin family
- length of signal sequence: 29 AA

2. SignalP
- SignalP predict Sec signal peptide (Sec/SPI), Lipoprotein signal peptide (Sec/SPII), Tat signal peptide (Tat/SPI) or no signal peptide (Other)
- Sec/SPI signal peptide is predicted with a probalility of 98.56%. 
- cleavage site between position 18 and 19: AYS-RG with a probalility of 74.8%
- serum albumin (appears in the blood plasma -> secreted protein)

3. CD48
- CD48 possess a GPI-anchor and a potential GPI-modification site at position 220 was found by the analysis
- outside: 225 AAs, transmembrane helix: 17 AAs, inside: 1
- yes! CD48 is an antigen and is found on the surface of several immune cells. However, CD48 does not have a transmembrane domain, but consists of a GPI anchor via a C-terminal domain. The GPI linkage is through residue 220.