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Proper choice of DNA barcodes is critical for accurate sample demultiplexing and maintaining next-generation sequencing performance. Selecting well-balanced DNA barcode sets ensures reliable demultiplexing and maximizes data quality.

To help find balanced and compatible barcode sets for the Illumina NGS experiments, the DNA Barcode Combination Finder tool was developed in our lab and is available online. DNA Barcode Combination Finder identifies optimal barcode combinations for Illumina sequencing experiments and ensures correct index color balancing for XLEAP SBS reagents on the NextSeq 1000/2000 and NovaSeq X/X Plus platforms.