High-Content Microscopy

    Equipment

    Perkin Elmer Operetta High-Content Imaging System (HCS)

    Automated image acquisition and image analysis on multi well plates ( 6 – 384 wells)

    Fluorescense (Dapi to far red) , transmitted light and digital-phase contrast imaging

    Objectives: 2x,10x high NA, 20x, 40x high NA,

    Live cell imaging with incorporated CO2 and temperature controls

    PhenoLOGIC Machine learning

    Hamony High-Content Analysis Software

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 237502929 (INST 93/803-1 -FUGG­)

    Perkin Elmer Operetta CLS High-Content Imaging System (HCS)

    New and improved version of the Operetta

    Air and water immersion: Objectives: 5x air, 10x air, 20x air, 20x water, 40x water, 63x water

    8 LED light source

    sCMOS camera (16 bit resolution)

    Automated image acquisition and image analysis on multi well plates (6 – 384 wells)

    Fluorescense (Dapi to far red), transmitted light and digital-phase contrast imaging

    Live cell imaging with incorporated CO2 and temperature controls

    PhenoLOGIC Machine learning

    Hamony High-Content Analysis Software

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 440766788 (INST 93/1023-1 -FUGG)

     

    Usage fee (per hour): project dependent, please inquire.

    Contact Person : Dr. Ursula Eilers, Dr. Christina Schülein-Völk

    Room DK04, Core Facility Functional Genomics

    Leica DM6000B upright microscope - LMD6500 Laser Microdissection system

    Objectives: 1.25x DRY, 5x Dry, 10x DRY, 20x DRY, 40x CORR, 40Lx CORR

    (5x, 10x, 20x and 40Lx are suitable for laser microdissection)

    Filter cubes: A4 (blue), L5 (green), Y5 (red)

    Camera: DFC295 (digital color camera), DFC360FX (for fluorescence microscopy)

    Usage fee (per hour): 20 € (internal Biocenter ), 35 € (external)

    Contact Person:  Angela Grün

    Room B322 (Biochemistry & Molecular Biology)

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 186135568 (INST 93/720-1 FUGG)

    Incucyte SX5 Live Cell Imaging System (Sartorius)

    Automated system for non-invasive,  image acquisition of living cells at user-defined time intervals

    Brightfield, green and red wavelength image acquisition

    Cell by cell software with advanced classification module

    The system is housed in an IncuSafe CO2 Incubator (Panasonic)

    Usage fee (per hour): project dependent, please inquire

    Contact Person:  Tobias Roth

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 506524377 (INST 93/1112-1 FUGG)

    Please note:

    The German Reserach Foundation (Deutsche Forschungsgemeinschaft / DFG) requests to achknowledge usage of DFG funded microscopes in publications with the according project code and proposes to use the phrase: „funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - project code“. You can find the project codes in the list above. Further details can be found in the user guidlines / Nutzerordnung.