High-Content Microscopy

    Equipment

    Perkin Elmer Operetta High-Content Imaging System (HCS)

    Automated image acquisition and image analysis on multi well plates ( 6 – 384 wells)

    Fluorescense (Dapi to far red) , transmitted light and digital-phase contrast imaging

    Objectives: 2x,10x high NA, 20x, 40x high NA,

    Live cell imaging with incorporated CO2 and temperature controls

    PhenoLOGIC Machine learning

    Hamony High-Content Analysis Software

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 237502929 (INST 93/803-1 -FUGG­)


    Perkin Elmer Operetta CLS High-Content Imaging System (HCS)

    New and improved version of the Operetta

    Air and water immersion: Objectives: 5x air, 10x air, 20x air, 20x water, 40x water, 63x water

    8 LED light source

    sCMOS camera (16 bit resolution)

    Automated image acquisition and image analysis on multi well plates (6 – 384 wells)

    Fluorescense (Dapi to far red), transmitted light and digital-phase contrast imaging

    Live cell imaging with incorporated CO2 and temperature controls

    PhenoLOGIC Machine learning

    Hamony High-Content Analysis Software

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 440766788 (INST 93/1023-1 -FUGG)

     

    Usage fee (per hour): project dependent, please inquire.

    Contact Person : Dr. Ursula EilersDr. Christina Schülein-Völk

    Room DK04, Core Facility Functional Genomics


    Leica DM6000B upright microscope - LMD6500 Laser Microdissection system

    Objectives: 1.25x DRY, 5x Dry, 10x DRY, 20x DRY, 40x CORR, 40Lx CORR

    (5x, 10x, 20x and 40Lx are suitable for laser microdissection)

    Filter cubes: A4 (blue), L5 (green), Y5 (red)

    Camera: DFC295 (digital color camera), DFC360FX (for fluorescence microscopy)

    Usage fee (per hour): 20 € (internal Biocenter ), 35 € (external)

    Contact Person:  Angela Grün

    Room B322 (Biochemistry & Molecular Biology)

    Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 186135568 (INST 93/720-1 FUGG)

    Please note:

    The German Reserach Foundation (Deutsche Forschungsgemeinschaft / DFG) requests to achknowledge usage of DFG funded microscopes in publications with the according project code and proposes to use the phrase: „funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - project code“. You can find the project codes in the list above. Further details can be found in the user guidlines / Nutzerordnung.