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    Zentrale Abteilung für Mikroskopie - Imaging Core Facility

    Christian Stigloher

    Prof. Dr. Christian Stigloher

    Group Leader
    Zentrale Abteilung für Mikroskopie
    Am Hubland
    97074 Würzburg
    Germany
    Building: Biozentrum
    Room: DK 16
    Bild: Prof. Dr. Christian Stigloher

    Christian Stigloher is a molecular cell biologist and electron microscopist. The main focus of his research have been neurons, from early development as progenitor cells to the final differentiated state with functional synapses. He studied biology at the University of Würzburg and Duke University, North Carolina, USA. Christian’s doctoral thesis project in the laboratory of Laure Bally-Cuif at the Helmholtz Center Munich and the Technical University Munich was focused on the molecular biology and cellular behavior of neural progenitor cells using zebrafish as model organism. He then went as postdoctoral fellow to Jean-Louis Bessereau’s laboratory at the Ecole Normale Superieure in Paris, France, where he changed topic and focused on to the nervous system of the nematode C. elegans as model for molecular and ultrastructural analysis of synaptic architecture and function. Since 2012 Christian Stigloher is Juniorprofessor and since 2017 Professor for Microscopy at the Biocenter of the Julius-Maximilians-University Würzburg.

    Christian is particularly interested in understanding the dynamic behavior and architecture of cells and to combine this information with the molecular factors at play. He used zebrafish as model to unravel molecular components of an early patterning process in the nervous system that separates the eye field from the telencephalic progenitor pool. Vertebrate brains crucially rely on neural progenitor pools as source of undifferentiated and proliferating cells during development and partly also throughout life-time. He then focused on the molecular processes regulating the neural progenitor pool at the midbrain-hindbrain boundary (MHB) where he participated in a project that discovered a novel microRNA mediated process that regulates the MHB progenitor pool. As postdoc he then went on to use the small nematode C. elegans as model organism to study structure and function of chemical synapses, fine structured cellular junctions that allow communication between neurons themselves and neurons and muscle cells. As postdoctoral-fellow he learned to apply advanced electron microscopy techniques such as high pressure freezing and established electron tomography as tool to study synaptic architecture at the nanoscale in 3D. A special interest of his research is to combine microscopy techniques in a so called correlated light and electron microscopy (CLEM) approach. Thereby one can profit from the advantages of both techniques, allowing access to ultrastructural information with the knowledge of the localization of molecular factors.

    2017

    • Kaltdorf, K.V., Schulze, K., Helmprobst, F., Kollmannsberger, P., Dandekar, T., Stigloher, C. (2017) “FIJI Macro 3D ART VeSElecT: 3D Automated Reconstruction Tool for Vesicle Structures of Electron Tomograms”, PLOS Computational Biology, 13(1), 1-21, available: https://doi.org/10.1371/journal.pcbi.1005317.
       

    2016

    • Markert, S.M., Britz, S., Proppert, S., Lang, M., Witvliet, D., Mulcahy, B., Sauer, M., Zhen, M., Bessereau, J.-L., Stigloher, C. (2016) “Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome”, Neurophotonics, 3(4), 041802, available: https://doi.org/10.1117%2F1.nph.3.4.041802.
       

    2011

    • Stigloher, C., Zhan, H., Zhen, M., Richmond, J., Bessereau, J.-L. (2011) “The Presynaptic Dense Projection of the Caenorhabiditis elegans Cholinergic Neuromuscular Junction Localizes Synaptic Vesicles at the Active Zone through SYD-2/Liprin and UNC-10/RIM-Dependent Interactions”, Journal of Neuroscience, 31(12), 4388--4396, available: http://www.jneurosci.org/content/31/12/4388.
       

    2008

    • Leucht, C., Stigloher, C., Wizenmann, A., Klafke, R., Folchert, A., Bally-Cuif, L. (2008) “MicroRNA-9 directs late organizer activity of the midbrain-hindbrain boundary”, Nature Neuroscience, 11, 641--, available: https://doi.org/10.1038/nn.2115.
       

    2006

    • Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhäuser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., Bally-Cuif, L. (2006) “Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3”, Development, 133(15), 2925--2935, available: http://dev.biologists.org/content/133/15/2925.
       

    2019

    • Scholz, N., Ehmann, N., Sachidanandan, D., Imig, C., Cooper, B.H., Jahn, O., Reim, K., Brose, N., Meyer, J., Lamberty, M., Altrichter, S., Bormann, A., Hallermann, S., Pauli, M., Heckmann, M., Stigloher, C., Langenhan, T., Kittel, R.J. (2019) “Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix”, J Cell Biol, 218(3), 1011-1026, available: https://www.ncbi.nlm.nih.gov/pubmed/30782781.
       

    2018

    • Segerer, G., Engelmann, D., Kaestner, A., Trötzmüller, M., Köfeler, H., Stigloher, C., Thiele, C., Jeanclos, E., Gohla, A. (2018) “A phosphoglycolate phosphatase/AUM-dependent link between triacylglycerol turnover and epidermal growth factor signaling”, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1863(6), 584 - 594, available: http://www.sciencedirect.com/science/article/pii/S138819811830043X.
       
    • Beer, K.B., Rivas-Castillo, J., Kuhn, K., Fazeli, G., Karmann, B., Nance, J.F., Stigloher, C., Wehman, A.M. (2018) “Extracellular vesicle budding is inhibited by redundant regulators of TAT-5 flippase localization and phospholipid asymmetry”, Proceedings of the National Academy of Sciences, 115(6), E1127--E1136, available: https://www.pnas.org/content/115/6/E1127.
       
    • D'Alessandro, M., Richard, M., Stigloher, C., Vincent, G., Boulin, T., Richmond, J.E., Bessereau, J.-L. (2018) “CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors”, eLife, 7(e39649), available: https://doi.org/10.7554/eLife.39649.
       
    • Böhm, J., Messerer, M., Müller, H.M., Scholz-Starke, J., Gradogna, A., Scherzer, S., Maierhofer, T., Bazihizina, N., Zhang, H., Stigloher, C., Ache, P., Al-Rasheid, K.A.S., Mayer, K.F.X., Shabala, S., Carpaneto, A., Haberer, G., Zhu, J.-K., Hedrich, R. (2018) “Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa, Current Biology, 28(19), 3075 - 3085.e7, available: http://www.sciencedirect.com/science/article/pii/S0960982218310492.
       
    • Spindler, M.-C., Helmprobst, F., Stigloher, C., Benavente, R. (2018) “EM Tomography of Meiotic LINC Complexes”, in Gundersen, G.G. and Worman, H.J., eds., The Linc Complex: Methods And Protocols, Springer New York: New York, NY, 3--15, available: https://doi.org/10.1007/978-1-4939-8691-0_1.
       
    • Kaltdorf, K.V., Theiss, M., Markert, S.M., Zhen, M., Dandekar, T., Stigloher, C., Kollmannsberger, P. (2018) “Automated classification of synaptic vesicles in electron tomograms of C. elegans using machine learning”, PLOS ONE, 13(10), 1-22, available: https://doi.org/10.1371/journal.pone.0205348.
       
    • Link, J., Paouneskou, D., Velkova, M., Daryabeigi, A., Laos, T., Labella, S., Barroso, C., Piñol, S.P., Montoya, A., Kramer, H., Woglar, A., Baudrimont, A., Markert, S.M., Stigloher, C., Martinez-Perez, E., Dammermann, A., Alsheimer, M., Zetka, M., Jantsch, V. (2018) “Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase”, Developmental Cell, 45(2), 212 - 225.e7, available: http://www.sciencedirect.com/science/article/pii/S1534580718302387.
       

    2017

    • Schieber, N.L., Machado, P., Markert, S.M., Stigloher, C., Schwab, Y., Steyer, A.M. (2017) “Chapter 4 - Minimal resin embedding of multicellular specimens for targeted FIB-SEM imaging”, in Müller-Reichert, T. and Verkade, P., eds., Correlative Light And Electron Microscopy Iii, Methods In Cell Biology, Academic Press, 69 - 83, available: http://www.sciencedirect.com/science/article/pii/S0091679X17300493.
       
    • Helmprobst, F., Lillesaar, C., Stigloher, C. (2017) “Expression of sept3, sept5a and sept5b in the Developing and Adult Nervous System of the Zebrafish (Danio rerio)”, Frontiers in Neuroanatomy, 11, available: https://doi.org/10.3389%2Ffnana.2017.00006.
       
    • Kaltdorf, K.V., Schulze, K., Helmprobst, F., Kollmannsberger, P., Dandekar, T., Stigloher, C. (2017) “FIJI Macro 3D ART VeSElecT: 3D Automated Reconstruction Tool for Vesicle Structures of Electron Tomograms”, PLOS Computational Biology, 13(1), 1-21, available: https://doi.org/10.1371/journal.pcbi.1005317.
       
    • García-Fernández, E., Koch, G., Wagner, R.M., Fekete, A., Stengel, S.T., Schneider, J., Mielich-Süss, B., Geibel, S., Markert, S.M., Stigloher, C., Lopez, D. (2017) “Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance”, Cell, 171(6), 1354 - 1367.e20, available: http://www.sciencedirect.com/science/article/pii/S0092867417311996.
       
    • Markert, S.M., Bauer, V., Muenz, T.S., Jones, N.G., Helmprobst, F., Britz, S., Sauer, M., Rössler, W., Engstler, M., Stigloher, C. (2017) “Chapter 2 - 3D subcellular localization with superresolution array tomography on ultrathin sections of various species”, in Müller-Reichert, T. and Verkade, P., eds., Correlative Light And Electron Microscopy Iii, Methods In Cell Biology, Academic Press, 21 - 47, available: http://www.sciencedirect.com/science/article/pii/S0091679X17300481.
       

    2016

    • Jahn, M.T., Markert, S.M., Ryu, T., Ravasi, T., Stigloher, C., Hentschel, U., Moitinho-Silva, L. (2016) “Shedding light on cell compartmentation in the candidate phylum Poribacteria by high resolution visualisation and transcriptional profiling”, Scientific Reports, 6, 35860--, available: https://doi.org/10.1038/srep35860.
       
    • Kupper, M., Stigloher, C., Feldhaar, H., Gross, R. (2016) “Distribution of the obligate endosymbiont Blochmannia floridanus and expression analysis of putative immune genes in ovaries of the carpenter ant Camponotus floridanus, Arthropod Structure & Development, 45(5), 475 - 487, available: http://www.sciencedirect.com/science/article/pii/S1467803916300329.
       
    • Markert, S.M., Britz, S., Proppert, S., Lang, M., Witvliet, D., Mulcahy, B., Sauer, M., Zhen, M., Bessereau, J.-L., Stigloher, C. (2016) “Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome”, Neurophotonics, 3(4), 041802, available: https://doi.org/10.1117%2F1.nph.3.4.041802.
       

    2015

    • Frank, B., Marcu, A., Petersen, A.L. de O.A., Weber, H., Stigloher, C., Mottram, J.C., Scholz, C.J., Schurigt, U. (2015) “Autophagic digestion of Leishmania major by host macrophages is associated with differential expression of BNIP3, CTSE, and the miRNAs miR-101c, miR-129, and miR-210”, Parasites & Vectors, 8:404, available: https://doi.org/10.1186/s13071-015-0974-3.
       
    • Helmprobst, F., Frank, M., Stigloher, C. (2015) “Presynaptic architecture of the larval zebrafish neuromuscular junction”, Journal of Comparative Neurology, 523(13), 1984-1997, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.23775.
       

    2014

    • Pinan-Lucarré, B., Tu, H., Pierron, M., Cruceyra, P.I., Zhan, H., Stigloher, C., Richmond, J.E., Bessereau, J.-L. (2014) C. elegans Punctin specifies cholinergic versus GABAergic identity of postsynaptic domains”, Nature, 511, 466-470, available: https://doi.org/10.1038/nature13313.
       
    • Zhan, H., Stanciauskas, R., Stigloher, C., Dizon, K.K., Jospin, M., Bessereau, J.-L., Pinaud, F. (2014) In vivo single-molecule imaging identifies altered dynamics of calcium channels in dystrophin-mutant C. elegans, Nature Communications, 5, 4974--, available: https://doi.org/10.1038/ncomms5974.
       
    • Różycka, M., Wojtas, M., Jakób, M., Stigloher, C., Grzeszkowiak, M., Mazur, M., Ożyhar, A. (2014) “Intrinsically Disordered and Pliable Starmaker-Like Protein from Medaka (Oryzias latipes) Controls the Formation of Calcium Carbonate Crystals”, PLOS ONE, 9(12), 1-36, available: https://doi.org/10.1371/journal.pone.0114308.
       

    2013

    • Hung, W.L., Hwang, C., Gao, S.B., Liao, E.H., Chitturi, J., Wang, Y., Li, H., Stigloher, C., Bessereau, J.-L., Zhen, M. (2013) “Attenuation of insulin signalling contributes to FSN-1-mediated regulation of synapse development”, The EMBO Journal, 32(12), 1745--1760, available: https://doi.org/10.1038%2Femboj.2013.91.
       

    2012

    • Boulin, T., Rapti, G., Briseño-Roa, L., Stigloher, C., Richmond, J.E., Paoletti, P., Bessereau, J.-L. (2012) “Positive modulation of a Cys-loop acetylcholine receptor by an auxiliary transmembrane subunit”, Nature Neuroscience, 15, 1374--, available: https://doi.org/10.1038/nn.3197.
       

    2011

    • Stigloher, C., Zhan, H., Zhen, M., Richmond, J., Bessereau, J.-L. (2011) “The Presynaptic Dense Projection of the Caenorhabiditis elegans Cholinergic Neuromuscular Junction Localizes Synaptic Vesicles at the Active Zone through SYD-2/Liprin and UNC-10/RIM-Dependent Interactions”, Journal of Neuroscience, 31(12), 4388--4396, available: http://www.jneurosci.org/content/31/12/4388.
       
    • Webb, K.J., Coolen, M., Gloeckner, C.J., Stigloher, C., Bahn, B., Topp, S., Ueffing, M., Bally-Cuif, L. (2011) “The Enhancer of split transcription factor Her8a is a novel dimerisation partner for Her3 that controls anterior hindbrain neurogenesis in zebrafish”, BMC Developmental Biology, 11:27, available: https://doi.org/10.1186/1471-213X-11-27.
       
    • Chapouton, P., Webb, K.J., Stigloher, C., Alunni, A., Adolf, B., Hesl, B., Topp, S., Kremmer, E., Bally-Cuif, L. (2011) “Expression of Hairy/enhancer of split genes in neural progenitors and neurogenesis domains of the adult zebrafish brain”, Journal of Comparative Neurology, 519(9), 1748-1769, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.22599.
       

    2009

    • Lillesaar, C., Stigloher, C., Tannhäuser, B., Wullimann, M.F., Bally-Cuif, L. (2009) “Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression”, Journal of Comparative Neurology, 512(2), 158-182, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.21887.
       

    2008

    • Topp, S., Stigloher, C., Komisarczuk, A.Z., Adolf, B., Becker, T.S., Bally-Cuif, L. (2008) “Fgf signaling in the zebrafish adult brain: Association of Fgf activity with ventricular zones but not cell proliferation”, Journal of Comparative Neurology, 510(4), 422-439, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.21802.
       
    • Komisarczuk, A.Z., Topp, S., Stigloher, C., Kapsimali, M., Bally-Cuif, L., Becker, T.S. (2008) “Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group”, Developmental Dynamics, 237(9), 2594-2603, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/dvdy.21689.
       
    • Ninkovic, J., Stigloher, C., Lillesaar, C., Bally-Cuif, L. (2008) “Gsk3β/PKA and Gli1 regulate the maintenance of neural progenitors at the midbrain-hindbrain boundary in concert with E(Spl) factor activity”, Development, 135(18), 3137--3148, available: http://dev.biologists.org/content/135/18/3137.
       
    • Leucht, C., Stigloher, C., Wizenmann, A., Klafke, R., Folchert, A., Bally-Cuif, L. (2008) “MicroRNA-9 directs late organizer activity of the midbrain-hindbrain boundary”, Nature Neuroscience, 11, 641--, available: https://doi.org/10.1038/nn.2115.
       
    • Stigloher, C., Chapouton, P., Adolf, B., Bally-Cuif, L. (2008) “Identification of neural progenitor pools by E(Spl) factors in the embryonic and adult brain”, Brain Research Bulletin, 75(2), 266 - 273, available: http://www.sciencedirect.com/science/article/pii/S0361923007003310.
       
    • Söker, T., Dalke, C., Puk, O., Floss, T., Becker, L., Bolle, I., Favor, J., Hans, W., Hölter, S.M., Horsch, M., Kallnik, M., Kling, E., Moerth, C., Schrewe, A., Stigloher, C., Topp, S., Gailus-Durner, V., Naton, B., Beckers, J., Fuchs, H., Ivandic, B., Klopstock, T., Schulz, H., Wolf, E., Wurst, W., Bally-Cuif, L., de Angelis, M.H., Graw, J. (2008) “Pleiotropic effects in Eya3knockout mice”, BMC Developmental Biology, 8(1), 118, available: https://doi.org/10.1186/1471-213X-8-118.
       

    2007

    • Lillesaar, C., Tannhäuser, B., Stigloher, C., Kremmer, E., Bally-Cuif, L. (2007) “The serotonergic phenotype is acquired by converging genetic mechanisms within the zebrafish central nervous system”, Developmental Dynamics, 236(4), 1072-1084, available: https://onlinelibrary.wiley.com/doi/abs/10.1002/dvdy.21095.
       

    2006

    • Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhäuser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., Bally-Cuif, L. (2006) “Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3”, Development, 133(15), 2925--2935, available: http://dev.biologists.org/content/133/15/2925.