Publications
2022
Daniel Solvie, Apoorva Baluapuri, Leonie Uhl, Daniel Fleischhauer, Theresa Endres, Dimitrios Papadopoulos, Amel Aziba, Abdallah Gaballa, Ivan Mikicic, Ekaterina Isaakova, Celeste Giansanti, Jennifer Jansen, Marvin Jungblut, Teresa Klein, Christina Schülein-Völk, Hans Maric, Sören Doose, Markus Sauer, Petra Beli, Andreas Rosenwald, Matthias Dobbelstein, Elmar Wolf*, Eilers* (2022) MYC multimers shield stalled replication forks from RNA polymerase. Nature doi: 10.1038/s41586-022-05469-4.
Otto C, Kastner C, Schmidt S, Uttinger K, Baluapuri A, Denk S, Rosenfeldt MT, Rosenwald A, Roehrig F, Ade CP, Schuelein-Voelk C, Diefenbacher ME, Germer CT, Wolf E, Eilers M, Wiegering A (2022) RNA polymerase I inhibition induces terminal differentiation, growth arrest and vulnerability to senolytics in colorectal cancer cells. Mol Oncol
Schwarz JD, Lukassen S, Bhandare P, Eing L, Snaebjörnsson MT, Cruz García Y, Kisker JP, Schulze A, Wolf E (2022)The glycolytic enzyme ALDOA and the exon junction complex protein RBM8A are regulators of ribosomal biogenesis. Frontiers in Cell and Developmental Biology doi: 10.3389/fcell.2022.954358
Bozilovic J, Eing L, Berger BT, Adhikari B, Weckesser J, Berner NB, Wilhelm S, Kuster B, Wolf E*, Knapp S* (2022)Novel, highly potent PROTACs targeting AURORA-A kinase. Current Research in Chemical Biology doi: 10.1016/j.crchbi.2022.100032.
Doffo J, Bamopoulos SA, Köse H, Orben F, Zang C, Pons M, den Dekker AT, Brouwer RWW, Baluapuri A, Habringer S, Reichert M, Illendula A, Krämer OH, Schick M, Wolf E, van IJcken WFJ, Esposito I, Keller U, Schneider G, Wirth M. NOXA expression drives synthetic lethality to RUNX1 inhibition in pancreatic cancer. PNAS. 2022, 119(9).
Riedel A, Helal M, Pedro L, Swietlik JJ, Shorthouse D, Schmitz W, Haas L, Young T, da Costa ASH, Davidson S, Bhandare P, Wolf E, Hall BA, Frezza C, Oskarsson T, Shields JD. Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma. Cancer Immunol Res. 2022, 10(4):482-497
Schick M, Zhang L, Maurer S, Maurer HC, Isaakaidis K,Schneider L, Patra U, Schunck K, Rohleder E, Hofstetter J, Baluapuri A,Scherger AK, Slotta-Huspenina J, Hettler F, Weber J, Engleitner T, Maresch R,Slawska J, Lewis R, Istvanffy R, Habringer S, Steiger K, Baiker A, OostendorpRAJ, Miething C, Lenhof HP, Bassermann F, Chapuy B, Wirth M, Wolf E, Rad R, Müller S, Keller U (2022)Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis andgenetic instability in diffuse large B-cell lymphoma. Nature Communications doi: 10.1038/s41467-021-27704-8
2021
57. Narain A, Bhandare P, Adhikari B, Backes S, Eilers M, Dölken L, Schlosser A, Erhard F, Baluapuri A, Wolf E, Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination, Molecular Cell, 2021, 10.1016/j.molcel.2021.06.016
56. Hartmann O, Reissland M, Maier CR, Fischer T, Prieto-Garcia C, Baluapuri A, Schwarz J, Schmitz W, Garrido-Rodriguez M, Pahor N, Davies CC, Bassermann F, Orian A, Wolf E, Schulze A, Calzado MA, Rosenfeldt MT, Diefenbacher ME, Implementation of CRISPR/Cas9 Genome Editing to Generate Murine Lung Cancer Models That Depict the Mutational Landscape of Human Disease. Front Cell Dev Biol. 2021,9
55. Dölle A, Adhikari B, Krämer A, Weckesser J, Berner N, Berger LM, Diebold M, Szewczyk MM, Barsyte-Lovejoy D, Arrowsmith CH, Gebel J, Löhr F, Dötsch V, Eilers M, Heinzlmeir S, Kuster B, Sotriffer C, Wolf E*, Knapp S*, Design, Synthesis, and Evaluation of WD-Repeat-Containing Protein 5 (WDR5) Degraders, Journal of Medicinal Chemistry, 2021, doi: 10.1021/acs.jmedchem.1c00146
54. Adhikari* B, Narain A, Wolf* E (2021) Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cells. STAR Protoc 2: 100949 *corresponding authors
53. Papadopoulos D, Solvie D, Baluapuri A, Endres, T, Ha S., Herold S, Kalb J, Giansanti C, Schülein-Völk C, Ade CP, Schneider C, Gaballa A, Vos S, Fischer U, Dobbelstein M, Wolf E, Eilers M (2021) MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription‑replication conflicts. Mol Cell https://doi.org/10.1016/j.molcel.2021.11.002
52. Krenz B, Gebhardt-Wolf A, Ade CP, Gaballa A, Roehrig F, Vendelova E, Baluapuri A, Eilers U, Gallant P, D'Artista L, Wiegering A, Gasteiger G, Rosenfeldt MT, Bauer S, Zender L, Wolf E, Eilers M: MYC- and MIZ1-Dependent Vesicular Transport of Double-Strand RNA Controls Immune Evasion in Pancreatic Ductal Adenocarcinoma. Cancer Research, 2021 81, 4242-4256
51. Sipol A, Hameister E, Xue B, Hofstetter J, Barenboim M, ÖllingerR, Jain G, Prexler C, Rubio RA, Baldauf MC, Franchina DG, Petry A, Schmäh J, ThielU, Gorlach A, Cario G, Brenner D, Richter G, Grünewald TGP, Rad R, Wolf E, RulandJ, Sorensen PH, Burdach SEG, MondoA Drives B-ALL Malignancy through EnhancedAdaptation to Metabolic Stress, Blood, 2021, DOI: 10.1182/blood.2020007932
50. Wolpaw AJ, Bayliss R, Büchel G, Dang CV, Eilers M, Gustafson WC, Hansen GM, Jura N, Knapp S, Lemmon MA, Levens D, Maris JM, Marmorstein R, Metallo SJ, Park JR, Penn LZ, Rape M, Roussel MF, Shokat KM, Tansey WP, Verba KA, Vos SM, Weiss WA, Wolf E, Mossé YP (2021) Drugging the 'undruggable' MYCN oncogenic transcription factor: Overcoming previous obstacles to impact childhood cancers, Cancer Research, doi: 10.1158/0008-5472.CAN-20-3108
2020
49. Adhikari B, Bozilovic J, Diebold M,Schwarz JD, Hofstetter J, Schröder M, Wanior M, Narain A, Vogt M, DudvarskiStankovic N, Baluapuri A, Schönemann L, Eing L, Bhandare P, Kuster B, SchlosserA, Heinzlmeir S, Sotriffer C, Knapp S* WolfE*, PROTAC-mediated degradation reveals a non-catalytic function ofAURORA-A kinase, Nature Chemical Biology, 2020, https://www.nature.com/articles/s41589-020-00652-y
48. Endres T, Solvie D, Heidelberger J ,Andrioletti V ,Baluapuri A ,Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, Zuber J, Beli P, Popov N, Wolf E, Gallant P, Eilers M: Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters, Molecular Cell, in press
47. Fröschel C, Komorek J, Attard A, Marsell A, Lopez-Arboleda WA, Le Berre J, Wolf E, Geldner N, Waller F, Korte A, Dröge-Laser W. Plant roots employ cell-layer-specific programs to respond to pathogenic and beneficial microbes, Cell Host Microbe, in press
46. Jessen C, Kreß JKC, Baluapuri A, Hufnagel A, Schmitz W,Kneitz S, Roth S, Marquardt A, Appenzeller S, Ade CP, Glutsch V, Wobser M,Friedmann-Angeli JP, Mosteo L, Goding CR, Schilling B, Geissinger E, Wolf E, Meierjohann S, The transcriptionfactor NRF2 enhances melanoma malignancy by blocking differentiation andinducing COX2 expression, Oncogene, 2020, doi:10.1038/s41388-020-01477-8
45. Rajeeve K, Vollmuth N, Janaki-Raman S, Wulff TF, Baluapuri A, Dejure FR, Huber C, Fink J, Schmalhofer M, Schmitz W, Sivadasan R, Wolf E, Eisenreich W, Schulze A, Seibel J, Rudel T: Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis. Nature Microbiology, 2020, doi: 10.1038/s41564-020-0762-5
44. Wolf E and Eilers M: Targeting MYC Proteins for Tumor Therapy, Annual Review of Cancer Biology, 2020, 4
43. Baluapuri, A., Wolf, E., Eilers, M. (2020) Target gene-independent functions of Myc oncoproteins. Nature Reviews Molecular Cell Biology, 2020
42. Cossa, G., Roeschert, I., Prinz, F., Baluapuri, A., R.S., V., Schülein-Völk, C., Chang, Y.-C., Ade, C. P., Mastrobuoni, G., Girard, C., Wortmann, L., Walz, S., Lührmann, R., Kempa, S., Kuster, B., Wolf, E., Mumberg, D., Eilers, M. (2020) Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. Molecular Cell, 2020
2019
41. Baluapuri A*, Hofstetter J*, Dudvarski Stankovic N, Endres T, Bhandare P, Vos SM, Adhikari B, Schwarz JD, Narain A, Vogt M, Wang S-Y, Düster R, Jung LA, Vanselow JT, Wiegering A, Geyer M, Maric HM, Gallant P, Walz S, Schlosser A, Cramer P, Eilers M, Wolf E: MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation, Molecular Cell, 2019, 74, https://doi.org/10.1016/j.molcel.2019.02.031 *joined first authors
40. Schmidt S, Gay D, Uthe FW … Wolf E, Strathdee D, Schulze A, Germer CT, Rosenwald A, Sansom OJ, Eilers M, Wiegering A: A MYC–GCN2–eIF2α negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer, Nature Cell Biology, (2019, doi:10.1038/s41556-019-0408-0
39. Herold S*, Kalb J*, Büchel G*,Ade CP, Baluapuri A, Xu J, Koster J, Solvie D, Carstensen A, Klotz C, Rodewald S, Schülein-Völk C,Dobbelstein M, Wolf E, Molenaar J, Versteeg R,Walz S, Eilers M: Recruitment of BRCA1 limits MYCN-driven accumulation of stalled RNA polymerase, Nature, 2019, 567
38. Dammert MA, Brägelmann J, Olsen RR, Böhm S, Monhasery N, Whitney CP, Chalishazar MD, Tumbrink HL, Guthrie MR, Klein S, Ireland AS, Ryan J, Schmitt A, Marx A, Ozretic L, Castiglione R, Lorenz C, Jachimowicz RD, Wolf E, Thomas RK, Poirier JT, Büttner R, Sen T, Byers LA, Reinhardt HC, Letai A, Oliver TG, Sos ML: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer, Nature Communications,2019, 10, doi: 10.1038/s41467-019-11371-x.
37. Annunziata I, van de Vlekkert D, Wolf E, Finkelstein D, Neale G, Machado E, Mosca R, Campos Y, Tillman H, Roussel MF, Andrew Weesner J, Ellen Fremuth L, Qiu X, Han MJ, Grosveld GC, d'Azzo A, MYC competes with MiT/TFE in regulating lysosomal biogenesis and autophagy through an epigenetic rheostat. Nature Communications, 2019, 10, doi: 10.1038/s41467-019-11568-0.
36. Pattschull G, Walz S, Gründl M, Schwab M, Rühl E, Baluapuri A, Cindric-Vranesic A, Kneitz S, Wolf E, Ade CP, Rosenwald A, von Eyss B, Gaubatz S: The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes. Cell Reports, 2019, 27
Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells. Neoplasia, 2019 (11):1110-1120
2018
34. Pedrotti L, Weiste C, Nägele T, Wolf E, Lorenzin F, DietrichK, Mair A, Weckwerth W, Teige M, Baena-González E, Dröge-Laser W (2018) Snf1-RELATEDKINASE1-Controlled C/S1-bZIP Signaling Activates Alternative MitochondrialMetabolic Pathways to Ensure Plant Survival in Extended Darkness. The Plant Cell
2017
33. Büchel, G., Carstensen, A., Mak, K.Y., Roeschert, I., Leen, E., Sumara, O., Hofstetter, J., Herold, S., Kalb, J., Baluapuri, A., Poon, E., Kwok, C., Chesler, L., Maric H. M., Rickman, D. S., Wolf, E., Bayliss, R., Walz, S., Eilers, M. Association with Aurora-A controls N-MYC-dependent promoter escape and pause release of RNA polymerase II during cell cycle (2017). Cell Reports in press
32. Gerlach J. M., Furrer M., Gallant M., Birkel D., Baluapuri A., Wolf E. and Gallant P. (2017) PAF1 complex component Leo1 helps recruit Drosophila Myc to promoters, PNAS doi: 10.1073/pnas.1705816114
31. Dejure, F.R., Royla, N., Herold, S., Kalb, J., Walz, S., Ade, C.P., Mastrobuoni, G., Vanselow, J., Schlosser, A., Wolf, E., Kempa, S., and Eilers, M. (2017) The 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels. EMBO J, Apr 13 doi:10.15252/embj.201796662. [Epub ahead of print]
30. Stopp S, Gründl M, Fackler M, Malkmus J, Leone M, Naumann R, Frantz S, Wolf E, von Eyss B, Engel FB, Gaubatz S., Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development, PNAS, 2017; 114(30): 8029-8034.
2016
29. Lorenzin F, Benary U, Baluapuri A, Walz S, Jung LA, von Eyss B, Kisker C, Wolf J, Eilers M, Wolf E, Different promoter affinities account for specificity in MYC-dependent gene regulation, eLife, 2016, e15161
28. Vo BT*, Wolf E*, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz S, Murphy BL, Youn YH, Han YG, Eilers M*, Roussel MF*, The interaction of Myc with Miz1 defines medulloblastoma subgroup identity, Cancer Cell, 2016, 29, 5-16 *joined first and senior authors
27. Jung L A, Gebhardt A, Koelmel W, Ade CP, Walz S, Kuper J, von Eyss B, Letschert S, Redel C, d'Artista L, Biankin A, Zender L, Sauer M, Wolf E, Evan G, Kisker C, Eilers M, OmoMYC blunts promoter invasion by oncogenic MYC to inhibit gene expression characteristic of MYC-dependent tumors, Oncogene, 2016
26. King B, Boccalatte F, Moran-Crusio K, Wolf E, Wang J, Kayembe C, Lazaris C, Yu X, Aranda-Orgilles B, Lasorella A, Aifantis I, The ubiquitin ligase Huwe1 regulates the maintenance and lymphoid commitment of hematopoietic stem cells, Nature Immunology, 2016; 17
25. Jaenicke LA, von Eyss B, Carstensen A, Wolf E, Xu W, Greifenberg A, Geyer M, Eilers M, and Popov N (2016). Ubiquitin-dependent turnover of MYC antagonizes MYC/PAF1C complex accumulation to drive transcriptional elongation. Mol Cell, 2016 Jan 7;61(1):54-67. doi: 10.1016/j.molcel.2015.11.007. Epub 2015 Dec 10.
2015
24. Küspert M, Murakawa Y, Schäffler K, Vanselow JT, Wolf E, Juranek S, Schlosser A, Landthaler M, Fischer U, LARP4B i an AU-rich sequence associated factor that promotes mRNA accumulation and translagtion, RNA, 2015
23. Herter EK, Stauch M, Gallant M, Wolf E, Raabe T, Gallant P,snoRNAs are a novel class of biologically relevant Myc targets, BMC Biology, 2015, 13
22. Wiese KE, Haikala HM, von Eyss B, Wolf E, Esnault C, Rosenwald A, Treismann R, Klefström J, Eilers M: Repression of SRF target genes is critical for Myc-dependent apoptosis of epithelial cells, EMBO J, 2015
21. Wolf E, Lin CY, Eilers E, Levens DL, Taming of the beast: Shaping Myc-dependent amplification, Trends in Cell Biology, 2015, 25
20. Weber D, Heisig J, Kneitz S, Wolf E, Eilers M, Gessler M: Mechanisms of epigenetic and cell-type specific regulation of Hey target genes in ES cells and cardiomyocytes, Journal of Molecular and Cellular Cardiology, 2015, 79, 79-88
19. Elkon R, Loayza-Puch F, Korkmaz G, Lopes R, van Breugel PC, Bleijerveld OB, Altelaar AF, Wolf E, Lorenzin F, Eilers M, Agami R. EMBO reports. 2015; 16(12):1723-36.
2014
18. Schülein-Völk C, Wolf E, Zhu J, Xu W, Taranets L, Hellmann A, Diefenbacher M, Behrens A, Eilers M, Popov N: Dual regulation of Fbw7 function and oncogenic transformation by Usp28, Cell Reports, 2014, 9, 1099-1109
17. Muthalagu N, Junttila MR, Wiese KE, Wolf E, Morton J, Bauer B, Evan GI, Eilers M, Murphy DJ: BIM Is the Primary Mediator of MYC-Induced Apoptosis in Multiple Solid Tissues, Cell Reports, 2014, 8, 1347-1353
16. Walz S, Lorenzin F, Morton J, Wiese KE, von Eyss B, Herold S, Rycak L, Dumay-Odelot H, Karim S, Bartkuhn M, Roels F, Wuestefeld T, Fischer M, Teichmann M, Zender L, Wei CL, Sansom O, Wolf E*, Eilers M*: Activation and repression by oncogenic Myc shape tumour-specific gene expression profiles, Nature, 2014, 511, 483-487 *these authors contributed equally , shared senior-authorship
15. Baumgart S, Chen NM, Siveke JT, Koenig A, Zhang JS, Singh SK, Wolf E, Bartkuhn M, Esposito I, Heßmann E, Reinecke J, Nikorowitsch J, Brunner M, Singh G, Fernandez-Zapico ME, Smyrk T, Bamlet WR, Eilers M, Neesse A, Gress TM, Billadeau DD, Tuveson DA, Urrutia R, Ellenrieder V: Inflammation induced NFATc1-STAT3 Transcription Complex Promotes Pancreatic Cancer initiation by KrasG12D, Cancer Discovery, 2014, 4, 688-701
14. Sanz-Moreno A, Fuhrmann D, Wolf E, von Eyss B, Eilers M, Elsässer HP: Miz1 deficiency in the mammary gland causes a lactation defect by attenuated stat5 expression and phosphorylation, PLos One, 2014, 9, e89187
2013
13. Wiese KE, Walz S, von Eyss B, Wolf E, Athineos D, Sansom O, Eilers M: The role of MIZ-1 in MYC-dependent tumorigenesis, Cold Spring Harb Perspect Med, 2013, 3, a014290
12. Wolf E*, Gebhardt A*, Kawauchi D, Walz S, von Eyss B, Wagner N, Renninger C, Krohne G, Asan E, Roussel MF, Eilers M: Miz1 is required to maintain autophagic flux, Nature Communications, 2013, ncomms3535, *these authors contributed equally
2012
11. Wolf E, Kastner B, Lührmann R: Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex, RNA, 2012, 18, 1347-1357
10. Hönnemann J, Sanz-Moreno A, Wolf E, Eilers M, Elsässer HP: Miz1 Is a Critical Repressor of cdkn1a during Skin Tumorigenesis, PLoS One, 2012, 7, e34885
9. Heisig J, Weber D, Englberger E, Winkler A, Kneitz S, Sung WK, Wolf E, Eilers M, Wei CL, Gessler M: Target Gene Analysis by Microarrays and Chromatin Immunoprecipitation Identifies HEY Proteins as Highly Redundant bHLH Repressors, PLoS Genetics, 2012, 8, e1002728
2011
8. Agafonov DE, Deckert J, Wolf E, Odenwälder P, Bessonov S, Will CL, Urlaub H, Lührmann R: Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method, Molecular and cellular biology, 2011, 31, 2667-2682
2010
7. Golas MM, Sander B, Bessonov S, Grote M, Wolf E, Kastner B, Stark H, Lührmann R: 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core, Molecular Cell, 2010, 40, 927-938
6. Grote M, Wolf E, Will CL, Lemm I, Agafonov DE, Schomburg A, Fischle W, Urlaub H, Lührmann R: Molecular architecture of the human Prp19/CDC5L complex, Molecular and cellular biology, 2010, 30, 2105-2119
2009
5. Wolf E, Kastner B, Deckert J, Merz C, Stark H, Lührmann R: Exon, intron and splice site locations in the spliceosomal B complex, EMBO J, 2009, 28, 2283-2292
4. Herold N, Will CL, Wolf E, Kastner B, Urlaub H, Lührmann L: Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes, Molecular and cellular biology, 2009, 29, 281-301
2008
3. Häcker I, Sander B, Golas MM, Wolf E, Karagöz E, Kastner B, Stark H, Fabrizio P, Lührmann R: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy, Nature structural and molecular biology, 2008, 15, 1206-1212
2. Kastner B, Fischer N, Golas MM, Sander B, Dube P, Boehringer D, Hartmuth K, Deckert J, Hauer F, Wolf E, Uchtenhagen H, Urlaub H, Pörschke D, Lührmann R, Stark H: GraFix: Sample preparation for singleparticle electron cryomicroscopy, Nature Methods, 2008, 5, 53-55
2005
1. Cheng Y, Wolf E, Larvie M, Zak O, Aisen P, Grigorieff N, Harrison SC, Walz T: Single particle reconstructions of the Transferrin–Transferrin receptor complex obtained with different specimen preparation techniques, Journal of Molecular Biology, 2005, 355, 1048-1065
