Chair of Biochemistry and Molecular Biology



    Adhikari B, Bozilovic J, Diebold M,Schwarz JD, Hofstetter J, Schröder M, Wanior M, Narain A, Vogt M, DudvarskiStankovic N, Baluapuri A, Schönemann L, Eing L, Bhandare P, Kuster B, SchlosserA, Heinzlmeir S, Sotriffer C, Knapp S* WolfE*, PROTAC-mediated degradation reveals a non-catalytic function ofAURORA-A kinase, Nature Chemical Biology, 2020, https://www.nature.com/articles/s41589-020-00652-y

    Wolpaw AJ, Bayliss R, Büchel G, Dang CV, Eilers M, Gustafson WC, Hansen GM, Jura N, Knapp S, Lemmon MA, Levens D, Maris JM, Marmorstein R, Metallo SJ, Park JR, Penn LZ, Rape M, Roussel MF, Shokat KM, Tansey WP, Verba KA, Vos SM, Weiss WA, Wolf E, Mossé YP (2021) Drugging the 'undruggable' MYCN oncogenic transcription factor: Overcoming previous obstacles to impact childhood cancers, Cancer Research,  doi: 10.1158/0008-5472.CAN-20-3108

    Endres T, Solvie D, Heidelberger J ,Andrioletti V ,Baluapuri A ,Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, Zuber J, Beli P, Popov N, Wolf E, Gallant P, Eilers M: Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters, Molecular Cell, in press

    Fröschel C, Komorek J, Attard A, Marsell A, Lopez-Arboleda WA, Le Berre J, Wolf E, Geldner N, Waller F, Korte A, Dröge-Laser W. Plant roots employ cell-layer-specific programs to respond to pathogenic and beneficial microbes, Cell Host Microbe, in press

    Jessen C, Kreß JKC, Baluapuri A, Hufnagel A, Schmitz W,Kneitz S, Roth S, Marquardt A, Appenzeller S, Ade CP, Glutsch V, Wobser M,Friedmann-Angeli JP, Mosteo L, Goding CR, Schilling B, Geissinger E, Wolf E, Meierjohann S, The transcriptionfactor NRF2 enhances melanoma malignancy by blocking differentiation andinducing COX2 expression, Oncogene, 2020, doi:10.1038/s41388-020-01477-8

    Rajeeve K, Vollmuth N, Janaki-Raman S, Wulff TF, Baluapuri A, Dejure FR, Huber C, Fink J, Schmalhofer M, Schmitz W, Sivadasan R, Wolf E, Eisenreich W, Schulze A, Seibel J, Rudel T: Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis. Nature Microbiology, 2020, doi: 10.1038/s41564-020-0762-5

    Wolf E and Eilers M: Targeting MYC Proteins for Tumor Therapy, Annual Review of Cancer Biology, 2020, 4

    Baluapuri, A., Wolf, E., Eilers, M. (2020) Target gene-independent functions of Myc oncoproteins. Nature Reviews Molecular Cell Biology in press

    Cossa, G., Roeschert, I., Prinz, F., Baluapuri, A., R.S., V., Schülein-Völk, C., Chang, Y.-C., Ade, C. P., Mastrobuoni, G., Girard, C., Wortmann, L., Walz, S., Lührmann, R., Kempa, S., Kuster, B., Wolf, E., Mumberg, D., Eilers, M. (2020) Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. Molecular Cell in press


    Baluapuri A*, Hofstetter J*, Dudvarski Stankovic N, Endres T, Bhandare P, Vos SM, Adhikari B, Schwarz JD, Narain A, Vogt M, Wang S-Y, Düster R, Jung LA, Vanselow JT, Wiegering A, Geyer M, Maric HM, Gallant P, Walz S, Schlosser A, Cramer P, Eilers M, Wolf E: MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation, Molecular Cell, 2019, 74, https://doi.org/10.1016/j.molcel.2019.02.031 *joined first authors

    Schmidt S, Gay D, Uthe FW … Wolf E, Strathdee D, Schulze A, Germer CT, Rosenwald A, Sansom OJ, Eilers M, Wiegering A: A MYC–GCN2–eIF2α negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer, Nature Cell Biology, (2019, doi:10.1038/s41556-019-0408-0

    Herold S*, Kalb J*, Büchel G*,Ade CP, Baluapuri A, Xu J, Koster J, Solvie D, Carstensen A, Klotz C, Rodewald S, Schülein-Völk C,Dobbelstein M, Wolf E, Molenaar J, Versteeg R,Walz S, Eilers M: Recruitment of BRCA1 limits MYCN-driven accumulation of stalled RNA polymerase, Nature, 2019, 567

    Dammert MA, Brägelmann J, Olsen RR, Böhm S, Monhasery N, Whitney CP, Chalishazar MD, Tumbrink HL, Guthrie MR, Klein S, Ireland AS, Ryan J, Schmitt A, Marx A, Ozretic L, Castiglione R, Lorenz C, Jachimowicz RD, Wolf E, Thomas RK, Poirier JT, Büttner R, Sen T, Byers LA, Reinhardt HC, Letai A, Oliver TG, Sos ML: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer, Nature Communications,2019, 10, doi: 10.1038/s41467-019-11371-x.

    Annunziata I, van de Vlekkert D, Wolf E, Finkelstein D, Neale G, Machado E, Mosca R, Campos Y, Tillman H, Roussel MF, Andrew Weesner J, Ellen Fremuth L, Qiu X, Han MJ, Grosveld GC, d'Azzo A, MYC competes with MiT/TFE in regulating lysosomal biogenesis and autophagy through an epigenetic rheostat. Nature Communications, 2019, 10, doi: 10.1038/s41467-019-11568-0.

    Pattschull G, Walz S, Gründl M, Schwab M, Rühl E, Baluapuri A, Cindric-Vranesic A, Kneitz S, Wolf E, Ade CP, Rosenwald A, von Eyss B, Gaubatz S: The Myb-MuvB Complex Is Required for YAP-Dependent Transcription of Mitotic Genes. Cell Reports, 2019, 27

    Otto C, Schmidt S, Kastner C, Denk S, Kettler J, Müller N, Germer CT, Wolf E, Gallant P, Wiegering A. Targeting bromodomain-containing protein 4 (BRD4) inhibits MYC expression in colorectal cancer cells. Neoplasia, 2019 (11):1110-1120


    Pedrotti L, Weiste C, Nägele T, Wolf E, Lorenzin F, DietrichK, Mair A, Weckwerth W, Teige M, Baena-González E, Dröge-Laser W (2018) Snf1-RELATEDKINASE1-Controlled C/S1-bZIP Signaling Activates Alternative MitochondrialMetabolic Pathways to Ensure Plant Survival in Extended Darkness. The Plant Cell


    Büchel, G., Carstensen, A., Mak, K.Y., Roeschert, I., Leen, E., Sumara, O., Hofstetter, J., Herold, S., Kalb, J., Baluapuri, A., Poon, E., Kwok, C., Chesler, L., Maric H. M., Rickman, D. S., Wolf, E., Bayliss, R., Walz, S., Eilers, M. Association with Aurora-A controls N-MYC-dependent promoter escape and pause release of RNA polymerase II during cell cycle (2017). Cell Reports in press

    Gerlach J. M., Furrer M., Gallant M., Birkel D., Baluapuri A., Wolf E. and Gallant P. (2017) PAF1 complex component Leo1 helps recruit Drosophila Myc to promoters, PNAS doi: 10.1073/pnas.1705816114

    Dejure, F.R., Royla, N., Herold, S., Kalb, J., Walz, S., Ade, C.P., Mastrobuoni, G., Vanselow, J., Schlosser, A., Wolf, E., Kempa, S., and Eilers, M. (2017) The 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels. EMBO J, Apr 13 doi:10.15252/embj.201796662. [Epub ahead of print]

    Stopp S, Gründl M, Fackler M, Malkmus J, Leone M, Naumann R, Frantz S, Wolf E, von Eyss B, Engel FB, Gaubatz S., Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development, PNAS, 2017; 114(30): 8029-8034.


    Lorenzin F, Benary U, Baluapuri A, Walz S, Jung LA, von Eyss B, Kisker C, Wolf J, Eilers M, Wolf E, Different promoter affinities account for specificity in MYC-dependent gene regulation, eLife, 2016, e15161

    Vo BT*, Wolf E*, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz S, Murphy BL, Youn YH, Han YG, Eilers M*, Roussel MF*, The interaction of Myc with Miz1 defines medulloblastoma subgroup identity, Cancer Cell, 2016, 29, 5-16 *joined first and senior authors

    Jung L A, Gebhardt A, Koelmel W, Ade CP, Walz S, Kuper J, von Eyss B, Letschert S, Redel C, d'Artista L, Biankin A, Zender L, Sauer M, Wolf E, Evan G, Kisker C, Eilers M, OmoMYC blunts promoter invasion by oncogenic MYC to inhibit gene expression characteristic of MYC-dependent tumors, Oncogene, 2016

    King B, Boccalatte F, Moran-Crusio K, Wolf E, Wang J, Kayembe C, Lazaris C, Yu X, Aranda-Orgilles B, Lasorella A, Aifantis I, The ubiquitin ligase Huwe1 regulates the maintenance and lymphoid commitment of hematopoietic stem cells, Nature Immunology, 2016; 17

    Jaenicke LA, von Eyss B, Carstensen A, Wolf E, Xu W, Greifenberg A, Geyer M, Eilers M, and Popov N (2016). Ubiquitin-dependent turnover of MYC antagonizes MYC/PAF1C complex accumulation to drive transcriptional elongation. Mol Cell, 2016 Jan 7;61(1):54-67. doi: 10.1016/j.molcel.2015.11.007. Epub 2015 Dec 10.


    Küspert M, Murakawa Y, Schäffler K, Vanselow JT, Wolf E, Juranek S, Schlosser A, Landthaler M, Fischer U, LARP4B i an AU-rich sequence associated factor that promotes mRNA accumulation and translagtion, RNA, 2015

    Herter EK, Stauch M, Gallant M, Wolf E, Raabe T, Gallant P,snoRNAs are a novel class of biologically relevant Myc targets, BMC Biology, 2015, 13

    Wiese KE, Haikala HM, von Eyss B, Wolf E, Esnault C, Rosenwald A, Treismann R, Klefström J, Eilers M: Repression of SRF target genes is critical for Myc-dependent apoptosis of epithelial cells, EMBO J, 2015

    Wolf E, Lin CY, Eilers E, Levens DL, Taming of the beast: Shaping Myc-dependent amplification, Trends in Cell Biology, 2015, 25

    Weber D, Heisig J, Kneitz S, Wolf E, Eilers M, Gessler M: Mechanisms of epigenetic and cell-type specific regulation of Hey target genes in ES cells and cardiomyocytes, Journal of Molecular and Cellular Cardiology, 2015, 79, 79-88

    Elkon R, Loayza-Puch F, Korkmaz G, Lopes R, van Breugel PC, Bleijerveld OB, Altelaar AF, Wolf E, Lorenzin F, Eilers M, Agami R. EMBO reports. 2015; 16(12):1723-36.



    Schülein-Völk C, Wolf E, Zhu J, Xu W, Taranets L, Hellmann A, Diefenbacher M, Behrens A, Eilers M, Popov N: Dual regulation of Fbw7 function and oncogenic transformation by Usp28, Cell Reports, 2014, 9, 1099-1109

    Muthalagu N, Junttila MR, Wiese KE, Wolf E, Morton J, Bauer B, Evan GI, Eilers M, Murphy DJ: BIM Is the Primary Mediator of MYC-Induced Apoptosis in Multiple Solid Tissues, Cell Reports, 2014, 8, 1347-1353

    Walz S, Lorenzin F, Morton J, Wiese KE, von Eyss B, Herold S, Rycak L, Dumay-Odelot H, Karim S, Bartkuhn M, Roels F, Wuestefeld T, Fischer M, Teichmann M, Zender L, Wei CL, Sansom O, Wolf E*, Eilers M*: Activation and repression by oncogenic Myc shape tumour-specific gene expression profiles, Nature, 2014, 511, 483-487 *these authors contributed equally , shared senior-authorship

    Baumgart S, Chen NM, Siveke JT, Koenig A, Zhang JS, Singh SK, Wolf E, Bartkuhn M, Esposito I, Heßmann E, Reinecke J, Nikorowitsch J, Brunner M, Singh G, Fernandez-Zapico ME, Smyrk T, Bamlet WR, Eilers M, Neesse A, Gress TM, Billadeau DD, Tuveson DA, Urrutia R, Ellenrieder V: Inflammation induced NFATc1-STAT3 Transcription Complex Promotes Pancreatic Cancer initiation by KrasG12D, Cancer Discovery, 2014, 4,  688-701

    Sanz-Moreno A, Fuhrmann D, Wolf E, von Eyss B, Eilers M, Elsässer HP: Miz1 deficiency in the mammary gland causes a lactation defect by attenuated stat5 expression and phosphorylation, PLos One, 2014, 9, e89187


    Wiese KE, Walz S, von Eyss B, Wolf E, Athineos D, Sansom O, Eilers M: The role of MIZ-1 in MYC-dependent tumorigenesis, Cold Spring Harb Perspect Med, 2013, 3, a014290

    Wolf E*, Gebhardt A*, Kawauchi D, Walz S, von Eyss B, Wagner N, Renninger C, Krohne G, Asan E, Roussel MF, Eilers M: Miz1 is required to maintain autophagic flux, Nature Communications, 2013, ncomms3535, *these authors contributed equally


    Wolf E, Kastner B, Lührmann R: Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex, RNA, 2012, 18, 1347-1357

    Hönnemann J, Sanz-Moreno A, Wolf E, Eilers M, Elsässer HP: Miz1 Is a Critical Repressor of cdkn1a during Skin Tumorigenesis, PLoS One, 2012, 7, e34885

    Heisig J, Weber D, Englberger E, Winkler A, Kneitz S, Sung WK, Wolf E, Eilers M, Wei CL, Gessler M: Target Gene Analysis by Microarrays and Chromatin Immunoprecipitation Identifies HEY Proteins as Highly Redundant bHLH Repressors, PLoS Genetics, 2012, 8, e1002728


    Agafonov DE, Deckert J, Wolf E, Odenwälder P, Bessonov S, Will CL, Urlaub H, Lührmann R: Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method, Molecular and cellular biology, 2011, 31, 2667-2682


    Golas MM, Sander B, Bessonov S, Grote M, Wolf E, Kastner B, Stark H, Lührmann R: 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core, Molecular Cell, 2010, 40, 927-938

    Grote M, Wolf E, Will CL, Lemm I, Agafonov DE, Schomburg A, Fischle W, Urlaub H, Lührmann R: Molecular architecture of the human Prp19/CDC5L complex, Molecular and cellular biology, 2010, 30, 2105-2119


    Wolf E, Kastner B, Deckert J, Merz C, Stark H, Lührmann R: Exon, intron and splice site locations in the spliceosomal B complex, EMBO J, 2009, 28, 2283-2292

    Herold N, Will CL, Wolf E, Kastner B, Urlaub H, Lührmann L: Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes, Molecular and cellular biology, 2009, 29, 281-301


    Häcker I, Sander B, Golas MM, Wolf E, Karagöz E, Kastner B, Stark H, Fabrizio P, Lührmann R: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy, Nature structural and molecular biology, 2008, 15, 1206-1212

    Kastner B, Fischer N, Golas MM, Sander B, Dube P, Boehringer D, Hartmuth K, Deckert J, Hauer F, Wolf E, Uchtenhagen H, Urlaub H, Pörschke D, Lührmann R, Stark H: GraFix: Sample preparation for singleparticle electron cryomicroscopy, Nature Methods, 2008, 5, 53-55


    Cheng Y, Wolf E, Larvie M, Zak O, Aisen P, Grigorieff N, Harrison SC, Walz T: Single particle reconstructions of the Transferrin–Transferrin receptor complex obtained with different specimen preparation techniques, Journal of Molecular Biology, 2005, 355, 1048-1065