Priv.-Doz. Dr. Hannes Neuweiler

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Since 2010Lecturer & Group Leader, Department of Biotechnology & Biophysics, University of Würzburg, Germany
2006–2010 Marie Curie Fellow (6th framework programme of the EU) and Career Development Fellow in the laboratory of Prof. Alan Fersht, Medical Research Council Centre for Protein Engineering, Cambridge, United Kingdom
2004–2006 Postdoctoral Researcher, Department of Applied Laser Physics & Laser Spectroscopy, University of Bielefeld, Germany
2003–2004Postdoctoral Researcher, Department of Biophysical Chemistry, University of Heidelberg, Germany
1999–2002 PhD, Department of Physical Chemistry, University of Heidelberg, Germany
1995–1998Study of Chemistry and Diploma, University of Heidelberg, Germany
1993–1995Study of Chemistry, University of Frankfurt/Main, Germany

List of publications

2021[ to top ]
  • Warhead Reactivity Limits the Speed of Inhibition of the Cysteine Protease Rhodesain. Johe, Patrick; Jung, Sascha; Endres, Erik; Kersten, Christian; Zimmer, Collin; Ye, Weixiang; Sönnichsen, Carsten; Hellmich, Ute A.; Sotriffer, Christoph; Schirmeister, Tanja; Neuweiler, Hannes. In ACS Chem. Biol., 16(4), S. 661–670. American Chemical Society, 2021.
  • Structure, interdomain dynamics, and pH-dependent autoactivation of pro-rhodesain, the main lysosomal cysteine protease from African trypanosomes. Johé, Patrick; Jaenicke, Elmar; Neuweiler, Hannes; Schirmeister, Tanja; Kersten, Christian; Hellmich, Ute A. In Journal of Biological Chemistry, 296, S. 100565-. 2021.
  • Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics. Schubert, Jonathan; Schulze, Andrea; Prodromou, Chrisostomos; Neuweiler, Hannes. In Nature Communications, 12(1), S. 6964-. 2021.
  • Allosteric coupling of sub-millisecond clamshell motions in ionotropic glutamate receptor ligand-binding domains. Rajab, Suhaila; Bismin, Leah; Schwarze, Simone; Pinggera, Alexandra; Greger, Ingo H.; Neuweiler, Hannes. In Communications Biology, 4(1), S. 1056-. 2021.
2020[ to top ]
  • NMR assignments of a dynamically perturbed and dimerization inhibited N-terminal domain variant of a spider silk protein from E. australis. Goretzki, Benedikt; Heiby, Julia C.; Hacker, Carolin; Neuweiler, Hannes; Hellmich, Ute A. In Biomolecular NMR Assignments, 14(1), S. 67–71. 2020.
2019[ to top ]
  • Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk. Heiby, Julia C.; Goretzki, Benedikt; Johnson, Christopher M.; Hellmich, Ute A.; Neuweiler, Hannes. In Nature Communications, 10(1), S. 4378-. 2019.
  • On-target restoration of a split T cell-engaging antibody for precision immunotherapy. Banaszek, Agnes; Bumm, Thomas G. P.; Nowotny, Boris; Geis, Maria; Jacob, Kim; Wölfl, Matthias; Trebing, Johannes; Kucka, Kirstin; Kouhestani, Dina; Gogishvili, Tea; Krenz, Bastian; Lutz, Justina; Rasche, Leo; Hönemann, Dirk; Neuweiler, Hannes; Heiby, Julia C.; Bargou, Ralf C.; Wajant, Harald; Einsele, Hermann; Riethmüller, Gert; Stuhler, Gernot. In Nature Communications, 10(1), S. 5387-. 2019.
  • The conserved NxNNWHW motif in Aha-type co-chaperones modulates the kinetics of Hsp90 ATPase stimulation. Mercier, Rebecca; Wolmarans, Annemarie; Schubert, Jonathan; Neuweiler, Hannes; Johnson, Jill L.; LaPointe, Paul. In Nature Communications, 10(1), S. 1273-. 2019.
2018[ to top ]
  • Two-step self-assembly of a spider silk molecular clamp. Rat, Charlotte; Heiby, Julia C.; Bunz, Jessica P.; Neuweiler, Hannes. In Nature Communications, 9(1), S. 4779-. 2018.
2017[ to top ]
  • Conservation of folding and association within a family of spidroin N-terminal domains. Heiby, Julia C.; Rajab, Suhaila; Rat, Charlotte; Johnson, Christopher M.; Neuweiler, Hannes. In Scientific Reports, 7(1), S. 16789. Nature Publishing Group, 2017.
2016[ to top ]
  • Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism. Schulze, Andrea; Beliu, Gerti; Helmerich, Dominic A; Schubert, Jonathan; Pearl, Laurence H; Prodromou, Chrisostomos; Neuweiler, Hannes. In Nat Chem Biol, advance online publication. Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved., 2016.
2015[ to top ]
  • β-Structure within the Denatured State of the Helical Protein Domain BBL. Thukral, Lipi; Schwarze, Simone; Daidone, Isabella; Neuweiler, Hannes. In Journal of Molecular Biology. 2015.
2014[ to top ]
  • PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching. Sauer, Markus; Neuweiler, Hannes. In Methods Mol Biol - Fluorescence Spectroscopy and Microscopy, Bd. 1076, Y. Engelborghs, A. J. Visser (Hrsg.), S. 597–615. Humana Press, 2014.
  • Microsecond folding and domain motions of a spider silk protein structural switch. Ries, Julia; Schwarze, Simone; Johnson, Christopher M.; Neuweiler, Hannes. In Journal of the American Chemical Society, (ja), S. null. 2014.
2013[ to top ]
  • The N-terminal domains of spider silk proteins assemble ultrafast and protected from charge screening. Schwarze, Simone; Zwettler, Fabian U.; Johnson, Christopher M.; Neuweiler, Hannes. In Nat Commun, 4. Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved., 2013.
2012[ to top ]
  • Long-Range Modulation of Chain Motions within the Intrinsically Disordered Transactivation Domain of Tumor Suppressor p53. Lum, Jenifer K.; Neuweiler, Hannes; Fersht, Alan R. In Journal of the American Chemical Society, 134(3), S. 1617–1622. 2012.
2011[ to top ]
  • Backbone-Driven Collapse in Unfolded Protein Chains. Teufel, Daniel P.; Johnson, Christopher M.; Lum, Jenifer K.; Neuweiler, Hannes. In Journal of Molecular Biology, 409(2), S. 250–262. 2011.
  • Intrinsic Motions in the N-Terminal Domain of an Ionotropic Glutamate Receptor Detected by Fluorescence Correlation Spectroscopy. Jensen, Mette H.; Sukumaran, Madhav; Johnson, Christopher M.; Greger, Ingo H.; Neuweiler, Hannes. In Journal of Molecular Biology, 414(1), S. 96–105. 2011.
2010[ to top ]
  • Hydrogen-Bond Driven Loop-Closure Kinetics in Unfolded Polypeptide Chains. Daidone, Isabella; Neuweiler, Hannes; Doose, Sören; Sauer, Markus; Smith, Jeremy C. In PLOS COMPUTATIONAL BIOLOGY, 6(1). PUBLIC LIBRARY SCIENCE, 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA, 2010.
  • The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces. Arbely, Eyal; Neuweiler, Hannes; Sharpe, Timothy D.; Johnson, Christopher M.; Fersht, Alan R. In PROTEIN SCIENCE, 19(9), S. 1704–1713. JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA, 2010.
  • Carboxyl pK(a) Values and Acid Denaturation of BBL. Arbely, Eyal; Rutherford, Trevor J.; Neuweiler, Hannes; Sharpe, Timothy D.; Ferguson, Neil; Fersht, Alan R. In JOURNAL OF MOLECULAR BIOLOGY, 403(2), S. 313–327. ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND, 2010.
  • Kinetics of chain motions within a protein-folding intermediate. Neuweiler, Hannes; Banachewicz, Wiktor; Fersht, Alan R. In PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(51), S. 22106–22110. NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA, 2010.
2009[ to top ]
  • Reply to Campos et al.: Direct observation versus ambiguous kinetics and thermodynamics. Huang, Fang; Ying, Liming; Neuweiler, Hannes; Fersht, Alan R. In PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(52), S. E140. NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA, 2009.
  • Direct observation of ultrafast folding and denatured state dynamics in single protein molecules. Neuweiler, Hannes; Johnson, Christopher M.; Fersht, Alan R. In PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(44), S. 18569–18574. NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA, 2009.
  • Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. Doose, Sören; Neuweiler, Hannes; Sauer, Markus. In CHEMPHYSCHEM, 10(9-10, Sp. Iss. SI), S. 1389–1398. WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY, 2009.
  • The Folding Mechanism of BBL: Plasticity of Transition-State Structure Observed within an Ultrafast Folding Protein Family. Neuweiler, Hannes; Sharpe, Timothy D.; Rutherford, Trevor J.; Johnson, Christopher M.; Allen, Mark D.; Ferguson, Neil; Fersht, Alan R. In JOURNAL OF MOLECULAR BIOLOGY, 390(5), S. 1060–1073. ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND, 2009.
  • Downhill versus Barrier-Limited Folding of BBL 2: Mechanistic Insights from Kinetics of Folding Monitored by Independent Tryptophan Probes. Neuweiler, Hannes; Sharpe, Timothy D.; Johnson, Christopher M.; Teufel, Daniel P.; Ferguson, Neil; Fersht, Alan R. In JOURNAL OF MOLECULAR BIOLOGY, 387(4), S. 975–985. ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND, 2009.
2008[ to top ]
  • Changes in conformational dynamics of mRNA upon AtGRP7 binding studied by fluorescence correlation spectroscopy. Schuettpelz, Mark; Schoening, Jan C.; Doose, Sören; Neuweiler, Hannes; Peters, Elisabeth; Staiger, Dorothee; Sauer, Markus. In JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(29), S. 9507–9513. AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2008.
2007[ to top ]
  • A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer. Agaylan, Ashraf; Binder, Daniel; Sauer, Markus; Neuweiler, Hannes; Meyer, Oliver; Kiesewetter, Holger; Salama, Abdulgabar. In Cancer, 110(11), S. 2502–2506. Wiley Subscription Services, Inc., A Wiley Company, 2007.
  • Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy. Neuweiler, Hannes; Lollmann, Marc; Doose, Sören; Sauer, Markus. In JOURNAL OF MOLECULAR BIOLOGY, 365(3), S. 856–869. ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND, 2007.
  • Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix. Doose, Sören; Neuweiler, Hannes; Barsch, Hannes; Sauer, Markus. In PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(44), S. 17400–17405. NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA, 2007.
2006[ to top ]
  • UV Fluorescence Lifetime Imaging Microscopy: A Label-Free Method for Detection and Quantification of Protein Interactions. Schüttpelz, Mark; Müller, Christian; Neuweiler, Hannes; Sauer, Markus. In Analytical Chemistry, 78(3), S. 663–669. 2006.
  • The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy. Kim, J; Doose, S; Neuweiler, H; Sauer, M. In NUCLEIC ACIDS RESEARCH, 34(9), S. 2516–2527. OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND, 2006.
2005[ to top ]
  • A microscopic view of miniprotein folding: Enhanced folding efficiency through formation of an intermediate. Neuweiler, H; Doose, S; Sauer, M. In PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(46), S. 16650–16655. NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA, 2005.
  • A close look at fluorescence quenching of organic dyes by tryptophan. Doose, S; Neuweiler, H; Sauer, M. In CHEMPHYSCHEM, 6(11), S. 2277–2285. WILEY-V C H VERLAG GMBH, PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY, 2005.
  • Exploring life by single-molecule fluorescence spectroscopy. Neuweiler, H; Sauer, M. In ANALYTICAL CHEMISTRY, 77(9), S. 178A-185A. AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2005.
  • Monitoring antibody binding events in homogeneous solution by single-molecule fluorescence spectroscopy. Scheffler, S; Sauer, M; Neuweiler, H. In ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS, 219(5), S. 665–678. R OLDENBOURG VERLAG, LEKTORAT MINT, POSTFACH 80 13 60, D-81613 MUNICH, GERMANY, 2005.
2004[ to top ]
  • Using photoinduced charge transfer reactions to study conformational dynamics of biopolymers at the single-molecule level. Neuweiler, H; Sauer, M. In CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 5(3), S. 285–298. BENTHAM SCIENCE PUBL LTD, EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES, 2004.
2003[ to top ]
  • Fluorescence quenching of dyes by tryptophan: Interactions at atomic detail from combination of experiment and computer simulation. Vaiana, AC; Neuweiler, H; Schulz, A; Wolfrum, J; Sauer, M; Smith, JC. In JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 125(47), S. 14564–14572. AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2003.
  • Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level. Neuweiler, H; Schulz, A; Bohmer, M; Enderlein, J; Sauer, M. In JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 125(18), S. 5324–5330. AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, 2003.
2002[ to top ]
  • Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes. Neuweiler, Hannes; Schulz, Andreas; Vaiana, Andrea C.; Smith, Jeremy C.; Kaul, Sepp; Wolfrum, Jürgen; Sauer, Markus. In Angewandte Chemie International Edition, 41(24), S. 4769–4773. WILEY-VCH Verlag, 2002.