Priv.-Doz. Dr. Hannes Neuweiler

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Since 2010Lecturer & Group Leader, Department of Biotechnology & Biophysics, University of Würzburg, Germany
2006–2010 Marie Curie Fellow (6th framework programme of the EU) and Career Development Fellow in the laboratory of Prof. Alan Fersht, Medical Research Council Centre for Protein Engineering, Cambridge, United Kingdom
2004–2006 Postdoctoral Researcher, Department of Applied Laser Physics & Laser Spectroscopy, University of Bielefeld, Germany
2003–2004Postdoctoral Researcher, Department of Biophysical Chemistry, University of Heidelberg, Germany
1999–2002 PhD, Department of Physical Chemistry, University of Heidelberg, Germany
1995–1998Study of Chemistry and Diploma, University of Heidelberg, Germany
1993–1995Study of Chemistry, University of Frankfurt/Main, Germany

List of publications

2021[ to top ]
  • Allosteric coupling of sub-millisecond clamshell motions in ionotropic glutamate receptor ligand-binding domains Rajab, Suhaila; Bismin, Leah; Schwarze, Simone; Pinggera, Alexandra; Greger, Ingo H.; Neuweiler, Hannes in Communications Biology (2021). 4(1) 1056-.
  • Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics Schubert, Jonathan; Schulze, Andrea; Prodromou, Chrisostomos; Neuweiler, Hannes in Nature Communications (2021). 12(1) 6964-.
  • Warhead Reactivity Limits the Speed of Inhibition of the Cysteine Protease Rhodesain Johe, Patrick; Jung, Sascha; Endres, Erik; Kersten, Christian; Zimmer, Collin; Ye, Weixiang; Sönnichsen, Carsten; Hellmich, Ute A.; Sotriffer, Christoph; Schirmeister, Tanja; Neuweiler, Hannes in ACS Chem. Biol. (2021). 16(4) 661–670.
  • Structure, interdomain dynamics, and pH-dependent autoactivation of pro-rhodesain, the main lysosomal cysteine protease from African trypanosomes Johé, Patrick; Jaenicke, Elmar; Neuweiler, Hannes; Schirmeister, Tanja; Kersten, Christian; Hellmich, Ute A. in Journal of Biological Chemistry (2021). 296 100565-.
2020[ to top ]
  • NMR assignments of a dynamically perturbed and dimerization inhibited N-terminal domain variant of a spider silk protein from E. australis Goretzki, Benedikt; Heiby, Julia C.; Hacker, Carolin; Neuweiler, Hannes; Hellmich, Ute A. in Biomolecular NMR Assignments (2020). 14(1) 67–71.
2019[ to top ]
  • On-target restoration of a split T cell-engaging antibody for precision immunotherapy Banaszek, Agnes; Bumm, Thomas G. P.; Nowotny, Boris; Geis, Maria; Jacob, Kim; Wölfl, Matthias; Trebing, Johannes; Kucka, Kirstin; Kouhestani, Dina; Gogishvili, Tea; Krenz, Bastian; Lutz, Justina; Rasche, Leo; Hönemann, Dirk; Neuweiler, Hannes; Heiby, Julia C.; Bargou, Ralf C.; Wajant, Harald; Einsele, Hermann; Riethmüller, Gert; Stuhler, Gernot in Nature Communications (2019). 10(1) 5387-.
  • Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk Heiby, Julia C.; Goretzki, Benedikt; Johnson, Christopher M.; Hellmich, Ute A.; Neuweiler, Hannes in Nature Communications (2019). 10(1) 4378-.
  • The conserved NxNNWHW motif in Aha-type co-chaperones modulates the kinetics of Hsp90 ATPase stimulation Mercier, Rebecca; Wolmarans, Annemarie; Schubert, Jonathan; Neuweiler, Hannes; Johnson, Jill L.; LaPointe, Paul in Nature Communications (2019). 10(1) 1273-.
2018[ to top ]
  • Two-step self-assembly of a spider silk molecular clamp Rat, Charlotte; Heiby, Julia C.; Bunz, Jessica P.; Neuweiler, Hannes in Nature Communications (2018). 9(1) 4779-.
2017[ to top ]
  • Conservation of folding and association within a family of spidroin N-terminal domains Heiby, Julia C.; Rajab, Suhaila; Rat, Charlotte; Johnson, Christopher M.; Neuweiler, Hannes in Scientific Reports (2017). 7(1) 16789.
2016[ to top ]
  • Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism Schulze, Andrea; Beliu, Gerti; Helmerich, Dominic A; Schubert, Jonathan; Pearl, Laurence H; Prodromou, Chrisostomos; Neuweiler, Hannes in Nat Chem Biol (2016). advance online publication
2015[ to top ]
  • β-Structure within the Denatured State of the Helical Protein Domain BBL Thukral, Lipi; Schwarze, Simone; Daidone, Isabella; Neuweiler, Hannes in Journal of Molecular Biology (2015).
2014[ to top ]
  • Microsecond folding and domain motions of a spider silk protein structural switch Ries, Julia; Schwarze, Simone; Johnson, Christopher M.; Neuweiler, Hannes in Journal of the American Chemical Society (2014). (ja) null.
  • PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching Sauer, Markus; Neuweiler, Hannes in Methods Mol Biol - Fluorescence Spectroscopy and Microscopy, Y. Engelborghs, A. J. Visser (Hrsg.) (2014). (Bd. 1076) 597–615.
2013[ to top ]
  • The N-terminal domains of spider silk proteins assemble ultrafast and protected from charge screening Schwarze, Simone; Zwettler, Fabian U.; Johnson, Christopher M.; Neuweiler, Hannes in Nat Commun (2013). 4
2012[ to top ]
  • Long-Range Modulation of Chain Motions within the Intrinsically Disordered Transactivation Domain of Tumor Suppressor p53 Lum, Jenifer K.; Neuweiler, Hannes; Fersht, Alan R. in Journal of the American Chemical Society (2012). 134(3) 1617–1622.
2011[ to top ]
  • Intrinsic Motions in the N-Terminal Domain of an Ionotropic Glutamate Receptor Detected by Fluorescence Correlation Spectroscopy Jensen, Mette H.; Sukumaran, Madhav; Johnson, Christopher M.; Greger, Ingo H.; Neuweiler, Hannes in Journal of Molecular Biology (2011). 414(1) 96–105.
  • Backbone-Driven Collapse in Unfolded Protein Chains Teufel, Daniel P.; Johnson, Christopher M.; Lum, Jenifer K.; Neuweiler, Hannes in Journal of Molecular Biology (2011). 409(2) 250–262.
2010[ to top ]
  • Kinetics of chain motions within a protein-folding intermediate Neuweiler, Hannes; Banachewicz, Wiktor; Fersht, Alan R. in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010). 107(51) 22106–22110.
  • Carboxyl pK(a) Values and Acid Denaturation of BBL Arbely, Eyal; Rutherford, Trevor J.; Neuweiler, Hannes; Sharpe, Timothy D.; Ferguson, Neil; Fersht, Alan R. in JOURNAL OF MOLECULAR BIOLOGY (2010). 403(2) 313–327.
  • The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces Arbely, Eyal; Neuweiler, Hannes; Sharpe, Timothy D.; Johnson, Christopher M.; Fersht, Alan R. in PROTEIN SCIENCE (2010). 19(9) 1704–1713.
  • Hydrogen-Bond Driven Loop-Closure Kinetics in Unfolded Polypeptide Chains Daidone, Isabella; Neuweiler, Hannes; Doose, Sören; Sauer, Markus; Smith, Jeremy C. in PLOS COMPUTATIONAL BIOLOGY (2010). 6(1)
2009[ to top ]
  • Downhill versus Barrier-Limited Folding of BBL 2: Mechanistic Insights from Kinetics of Folding Monitored by Independent Tryptophan Probes Neuweiler, Hannes; Sharpe, Timothy D.; Johnson, Christopher M.; Teufel, Daniel P.; Ferguson, Neil; Fersht, Alan R. in JOURNAL OF MOLECULAR BIOLOGY (2009). 387(4) 975–985.
  • Direct observation of ultrafast folding and denatured state dynamics in single protein molecules Neuweiler, Hannes; Johnson, Christopher M.; Fersht, Alan R. in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009). 106(44) 18569–18574.
  • Reply to Campos et al.: Direct observation versus ambiguous kinetics and thermodynamics Huang, Fang; Ying, Liming; Neuweiler, Hannes; Fersht, Alan R. in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009). 106(52) E140.
  • The Folding Mechanism of BBL: Plasticity of Transition-State Structure Observed within an Ultrafast Folding Protein Family Neuweiler, Hannes; Sharpe, Timothy D.; Rutherford, Trevor J.; Johnson, Christopher M.; Allen, Mark D.; Ferguson, Neil; Fersht, Alan R. in JOURNAL OF MOLECULAR BIOLOGY (2009). 390(5) 1060–1073.
  • Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules Doose, Sören; Neuweiler, Hannes; Sauer, Markus in CHEMPHYSCHEM (2009). 10(9-10, Sp. Iss. SI) 1389–1398.
2008[ to top ]
  • Changes in conformational dynamics of mRNA upon AtGRP7 binding studied by fluorescence correlation spectroscopy Schuettpelz, Mark; Schoening, Jan C.; Doose, Sören; Neuweiler, Hannes; Peters, Elisabeth; Staiger, Dorothee; Sauer, Markus in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008). 130(29) 9507–9513.
2007[ to top ]
  • A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer Agaylan, Ashraf; Binder, Daniel; Sauer, Markus; Neuweiler, Hannes; Meyer, Oliver; Kiesewetter, Holger; Salama, Abdulgabar in Cancer (2007). 110(11) 2502–2506.
  • Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix Doose, Sören; Neuweiler, Hannes; Barsch, Hannes; Sauer, Markus in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007). 104(44) 17400–17405.
  • Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy Neuweiler, Hannes; Lollmann, Marc; Doose, Sören; Sauer, Markus in JOURNAL OF MOLECULAR BIOLOGY (2007). 365(3) 856–869.
2006[ to top ]
  • The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy Kim, J; Doose, S; Neuweiler, H; Sauer, M in NUCLEIC ACIDS RESEARCH (2006). 34(9) 2516–2527.
  • UV Fluorescence Lifetime Imaging Microscopy: A Label-Free Method for Detection and Quantification of Protein Interactions Schüttpelz, Mark; Müller, Christian; Neuweiler, Hannes; Sauer, Markus in Analytical Chemistry (2006). 78(3) 663–669.
2005[ to top ]
  • Monitoring antibody binding events in homogeneous solution by single-molecule fluorescence spectroscopy Scheffler, S; Sauer, M; Neuweiler, H in ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS (2005). 219(5) 665–678.
  • A microscopic view of miniprotein folding: Enhanced folding efficiency through formation of an intermediate Neuweiler, H; Doose, S; Sauer, M in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005). 102(46) 16650–16655.
  • Exploring life by single-molecule fluorescence spectroscopy Neuweiler, H; Sauer, M in ANALYTICAL CHEMISTRY (2005). 77(9) 178A-185A.
  • A close look at fluorescence quenching of organic dyes by tryptophan Doose, S; Neuweiler, H; Sauer, M in CHEMPHYSCHEM (2005). 6(11) 2277–2285.
2004[ to top ]
  • Using photoinduced charge transfer reactions to study conformational dynamics of biopolymers at the single-molecule level Neuweiler, H; Sauer, M in CURRENT PHARMACEUTICAL BIOTECHNOLOGY (2004). 5(3) 285–298.
2003[ to top ]
  • Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level Neuweiler, H; Schulz, A; Bohmer, M; Enderlein, J; Sauer, M in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003). 125(18) 5324–5330.
  • Fluorescence quenching of dyes by tryptophan: Interactions at atomic detail from combination of experiment and computer simulation Vaiana, AC; Neuweiler, H; Schulz, A; Wolfrum, J; Sauer, M; Smith, JC in JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003). 125(47) 14564–14572.
2002[ to top ]
  • Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes Neuweiler, Hannes; Schulz, Andreas; Vaiana, Andrea C.; Smith, Jeremy C.; Kaul, Sepp; Wolfrum, Jürgen; Sauer, Markus in Angewandte Chemie International Edition (2002). 41(24) 4769–4773.