Priv.-Doz. Dr. Hannes Neuweiler


Since 2010Lecturer & Group Leader, Department of Biotechnology & Biophysics, University of Würzburg, Germany
2006–2010 Marie Curie Fellow (6th framework programme of the EU) and Career Development Fellow in the laboratory of Prof. Alan Fersht, Medical Research Council Centre for Protein Engineering, Cambridge, United Kingdom
2004–2006 Postdoctoral Researcher, Department of Applied Laser Physics & Laser Spectroscopy, University of Bielefeld, Germany
2003–2004Postdoctoral Researcher, Department of Biophysical Chemistry, University of Heidelberg, Germany
1999–2002 PhD, Department of Physical Chemistry, University of Heidelberg, Germany
1995–1998Study of Chemistry and Diploma, University of Heidelberg, Germany
1993–1995Study of Chemistry, University of Frankfurt/Main, Germany

List of publications

2021[ to top ]
  • Rajab, S., Bismin, L., Schwarze, S., Pinggera, A., Greger, I.H., Neuweiler, H.: Allosteric coupling of sub-millisecond clamshell motions in ionotropic glutamate receptor ligand-binding domains. Communications Biology. 4, 1056- (2021).
  • Schubert, J., Schulze, A., Prodromou, C., Neuweiler, H.: Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics. Nature Communications. 12, 6964- (2021).
  • Johe, P., Jung, S., Endres, E., Kersten, C., Zimmer, C., Ye, W., Sönnichsen, C., Hellmich, U.A., Sotriffer, C., Schirmeister, T., Neuweiler, H.: Warhead Reactivity Limits the Speed of Inhibition of the Cysteine Protease Rhodesain. ACS Chem. Biol. 16, 661–670 (2021).
  • Johé, P., Jaenicke, E., Neuweiler, H., Schirmeister, T., Kersten, C., Hellmich, U.A.: Structure, interdomain dynamics, and pH-dependent autoactivation of pro-rhodesain, the main lysosomal cysteine protease from African trypanosomes. Journal of Biological Chemistry. 296, 100565- (2021).
2020[ to top ]
  • Goretzki, B., Heiby, J.C., Hacker, C., Neuweiler, H., Hellmich, U.A.: NMR assignments of a dynamically perturbed and dimerization inhibited N-terminal domain variant of a spider silk protein from E. australis. Biomolecular NMR Assignments. 14, 67–71 (2020).
2019[ to top ]
  • Banaszek, A., Bumm, T.G.P., Nowotny, B., Geis, M., Jacob, K., Wölfl, M., Trebing, J., Kucka, K., Kouhestani, D., Gogishvili, T., Krenz, B., Lutz, J., Rasche, L., Hönemann, D., Neuweiler, H., Heiby, J.C., Bargou, R.C., Wajant, H., Einsele, H., Riethmüller, G., Stuhler, G.: On-target restoration of a split T cell-engaging antibody for precision immunotherapy. Nature Communications. 10, 5387- (2019).
  • Heiby, J.C., Goretzki, B., Johnson, C.M., Hellmich, U.A., Neuweiler, H.: Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk. Nature Communications. 10, 4378- (2019).
  • Mercier, R., Wolmarans, A., Schubert, J., Neuweiler, H., Johnson, J.L., LaPointe, P.: The conserved NxNNWHW motif in Aha-type co-chaperones modulates the kinetics of Hsp90 ATPase stimulation. Nature Communications. 10, 1273- (2019).
2018[ to top ]
  • Rat, C., Heiby, J.C., Bunz, J.P., Neuweiler, H.: Two-step self-assembly of a spider silk molecular clamp. Nature Communications. 9, 4779- (2018).
2017[ to top ]
  • Heiby, J.C., Rajab, S., Rat, C., Johnson, C.M., Neuweiler, H.: Conservation of folding and association within a family of spidroin N-terminal domains. Scientific Reports. 7, 16789 (2017).
2016[ to top ]
  • Schulze, A., Beliu, G., Helmerich, D.A., Schubert, J., Pearl, L.H., Prodromou, C., Neuweiler, H.: Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism. Nat Chem Biol. advance online publication, (2016).
2015[ to top ]
  • Thukral, L., Schwarze, S., Daidone, I., Neuweiler, H.: β-Structure within the Denatured State of the Helical Protein Domain BBL. Journal of Molecular Biology. (2015).
2014[ to top ]
  • Ries, J., Schwarze, S., Johnson, C.M., Neuweiler, H.: Microsecond folding and domain motions of a spider silk protein structural switch. Journal of the American Chemical Society. null (2014).
  • Sauer, M., Neuweiler, H.: PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching. In: Engelborghs, Y. und Visser, A.J. (hrsg.) Methods Mol Biol - Fluorescence Spectroscopy and Microscopy. S. 597–615. Humana Press (2014).
2013[ to top ]
  • Schwarze, S., Zwettler, F.U., Johnson, C.M., Neuweiler, H.: The N-terminal domains of spider silk proteins assemble ultrafast and protected from charge screening. Nat Commun. 4, (2013).
2012[ to top ]
  • Lum, J.K., Neuweiler, H., Fersht, A.R.: Long-Range Modulation of Chain Motions within the Intrinsically Disordered Transactivation Domain of Tumor Suppressor p53. Journal of the American Chemical Society. 134, 1617–1622 (2012).
2011[ to top ]
  • Jensen, M.H., Sukumaran, M., Johnson, C.M., Greger, I.H., Neuweiler, H.: Intrinsic Motions in the N-Terminal Domain of an Ionotropic Glutamate Receptor Detected by Fluorescence Correlation Spectroscopy. Journal of Molecular Biology. 414, 96–105 (2011).
  • Teufel, D.P., Johnson, C.M., Lum, J.K., Neuweiler, H.: Backbone-Driven Collapse in Unfolded Protein Chains. Journal of Molecular Biology. 409, 250–262 (2011).
2010[ to top ]
  • Neuweiler, H., Banachewicz, W., Fersht, A.R.: Kinetics of chain motions within a protein-folding intermediate. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 107, 22106–22110 (2010).
  • Arbely, E., Rutherford, T.J., Neuweiler, H., Sharpe, T.D., Ferguson, N., Fersht, A.R.: Carboxyl pK(a) Values and Acid Denaturation of BBL. JOURNAL OF MOLECULAR BIOLOGY. 403, 313–327 (2010).
  • Arbely, E., Neuweiler, H., Sharpe, T.D., Johnson, C.M., Fersht, A.R.: The human peripheral subunit-binding domain folds rapidly while overcoming repulsive Coulomb forces. PROTEIN SCIENCE. 19, 1704–1713 (2010).
  • Daidone, I., Neuweiler, H., Doose, S., Sauer, M., Smith, J.C.: Hydrogen-Bond Driven Loop-Closure Kinetics in Unfolded Polypeptide Chains. PLOS COMPUTATIONAL BIOLOGY. 6, (2010).
2009[ to top ]
  • Neuweiler, H., Sharpe, T.D., Johnson, C.M., Teufel, D.P., Ferguson, N., Fersht, A.R.: Downhill versus Barrier-Limited Folding of BBL 2: Mechanistic Insights from Kinetics of Folding Monitored by Independent Tryptophan Probes. JOURNAL OF MOLECULAR BIOLOGY. 387, 975–985 (2009).
  • Neuweiler, H., Johnson, C.M., Fersht, A.R.: Direct observation of ultrafast folding and denatured state dynamics in single protein molecules. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106, 18569–18574 (2009).
  • Huang, F., Ying, L., Neuweiler, H., Fersht, A.R.: Reply to Campos et al.: Direct observation versus ambiguous kinetics and thermodynamics. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106, E140 (2009).
  • Neuweiler, H., Sharpe, T.D., Rutherford, T.J., Johnson, C.M., Allen, M.D., Ferguson, N., Fersht, A.R.: The Folding Mechanism of BBL: Plasticity of Transition-State Structure Observed within an Ultrafast Folding Protein Family. JOURNAL OF MOLECULAR BIOLOGY. 390, 1060–1073 (2009).
  • Doose, S., Neuweiler, H., Sauer, M.: Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules. CHEMPHYSCHEM. 10, 1389–1398 (2009).
2008[ to top ]
  • Schuettpelz, M., Schoening, J.C., Doose, S., Neuweiler, H., Peters, E., Staiger, D., Sauer, M.: Changes in conformational dynamics of mRNA upon AtGRP7 binding studied by fluorescence correlation spectroscopy. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 130, 9507–9513 (2008).
2007[ to top ]
  • Agaylan, A., Binder, D., Sauer, M., Neuweiler, H., Meyer, O., Kiesewetter, H., Salama, A.: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer. Cancer. 110, 2502–2506 (2007).
  • Doose, S., Neuweiler, H., Barsch, H., Sauer, M.: Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 104, 17400–17405 (2007).
  • Neuweiler, H., Lollmann, M., Doose, S., Sauer, M.: Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy. JOURNAL OF MOLECULAR BIOLOGY. 365, 856–869 (2007).
2006[ to top ]
  • Kim, J., Doose, S., Neuweiler, H., Sauer, M.: The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy. NUCLEIC ACIDS RESEARCH. 34, 2516–2527 (2006).
  • Schüttpelz, M., Müller, C., Neuweiler, H., Sauer, M.: UV Fluorescence Lifetime Imaging Microscopy: A Label-Free Method for Detection and Quantification of Protein Interactions. Analytical Chemistry. 78, 663–669 (2006).
2005[ to top ]
  • Scheffler, S., Sauer, M., Neuweiler, H.: Monitoring antibody binding events in homogeneous solution by single-molecule fluorescence spectroscopy. ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY \& CHEMICAL PHYSICS. 219, 665–678 (2005).
  • Neuweiler, H., Doose, S., Sauer, M.: A microscopic view of miniprotein folding: Enhanced folding efficiency through formation of an intermediate. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 102, 16650–16655 (2005).
  • Neuweiler, H., Sauer, M.: Exploring life by single-molecule fluorescence spectroscopy. ANALYTICAL CHEMISTRY. 77, 178A-185A (2005).
  • Doose, S., Neuweiler, H., Sauer, M.: A close look at fluorescence quenching of organic dyes by tryptophan. CHEMPHYSCHEM. 6, 2277–2285 (2005).
2004[ to top ]
  • Neuweiler, H., Sauer, M.: Using photoinduced charge transfer reactions to study conformational dynamics of biopolymers at the single-molecule level. CURRENT PHARMACEUTICAL BIOTECHNOLOGY. 5, 285–298 (2004).
2003[ to top ]
  • Neuweiler, H., Schulz, A., Bohmer, M., Enderlein, J., Sauer, M.: Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125, 5324–5330 (2003).
  • Vaiana, A., Neuweiler, H., Schulz, A., Wolfrum, J., Sauer, M., Smith, J.: Fluorescence quenching of dyes by tryptophan: Interactions at atomic detail from combination of experiment and computer simulation. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125, 14564–14572 (2003).
2002[ to top ]
  • Neuweiler, H., Schulz, A., Vaiana, A.C., Smith, J.C., Kaul, S., Wolfrum, J., Sauer, M.: Detection of Individual p53-Autoantibodies by Using Quenched Peptide-Based Molecular Probes. Angewandte Chemie International Edition. 41, 4769–4773 (2002).