With the CRISPR/Cas9-system you can modify the genome of human kidney cells or murine embryonic stem cells in a specific and precise way. The impact of this introduced mutation on the cells’ differentiation potential can then be investigated with a plethora of biochemical methods. For instance, you can analyze the expression of specific genes with quantitative real-time PCR or Western Blot.
Furthermore, through in vitro cultures of human Wilms tumor cells and their genome sequencing, you can help to contribute to a better understanding of this disease and thus, to finding effective treatments in the future.
- Cloning of a homologous recombination vector to introduce an inducible tumor-specific duplication of BCOR
- Differentiation alterations of Embryoid Bodies after the C-terminal deletion in the BCOR gene