Light Microscopy

Leica TCS SP8 upright, fixed stage - Confocal Laser Scanning Microscope with MP

Objectives: Revolver: 10x WATER, 20x IMM, 63x GLYC; Slider: 25x WATER

Laser Lines: VIS: 488nm, 552nm, 638nm; MP (Insight DeepSee Dual, SpectraPhysics): 680-1300nm (tunable), 1040nm 

Notch filter for VIS, Shortpass filter for MP

Detectors: Internal (for VIS): 2x PMT, 1x HyD; External (for MP): 2x PMT, 2x HyD

Usage fee (per hour) 20€ (internal BIOC.) 35€ (external)

Contact Person: K. Grübel, T. Münz, C. Groh

Room D103, Zoology II

Leica SP2 inverted microscope configuration - Confocal Laser Scanning Microscope

Objectives: 10x DRY, 20x IMM, 40x OIL, 63x OIL

Laser-Lines: UV: 405nm, VIS: 458nm, 476nm, 488nm, 496nm, 514nm, 561nm, 633nm 

AOBS (Acousto Optical Beam Splitter)

Detectors: 3x PMT, 1x PMT(Trans)

Usage fee (per hour)  15€ (internal BIOC.) 25€ (external)

Contact Person: Christian Stigloher

Room CK01

Leica SP8 (DM6000) upright microscope configuration - Confocal Laser Scanning Microscope

Objectives: 10x DRY, 20x IMM, 63x GLYC

Laser-Lines: Whitelaser,  UV: 405nm, VIS: 458nm, 476nm, 488nm, 496nm, 514nm,

AOBS (Acousto Optical Beam Splitter)

Detectors: 2x PMT, 1x PMT(Trans), 2x HyD

Usage fee (per hour)  20€ (internal BIOC.) 35€ (external)

Contact Person: Dirk Rieger

Room CK01

Zeiss Elyra S.1 SIM - Super Resolution Microscope

Objectives: 10x DRY, 63x OIL

Laser-Lines: UV: 405nm, VIS: 488nm, 561nm, 642nm

sCMOS-Camera: PCO Edge 5.5

Usage fee (per hour)  25€ (internal BIOC.) 40€ (external)

Contact Person: Marcus Behringer

Room CK01

Wide-Field Live Cell Imaging Setup - Zeiss Axio Examiner.D1 / Visitron Systems

Objectives: 20x WATER, 40x WATER, 100x OIL

Camera: 2x PCO Edge

Usage fee (per hour)  10€ (internal BIOC.) 15€ (external)

Contact Person: Christian Wegener

Room DK28

Perkin Elmer Operetta High-Content Imaging System (HCS)

Automated image acquisition and image analysis on multi well plates ( 6 – 384 wells)

Fluorescense (Dapi to far red) , transmitted light and digital-phase contrast imaging

Objectives: 2x,10x high NA, 20x, 40x high NA,

Live cell imaging with incorporated CO2 and temperature controls

PhenoLOGIC Machine learning

Hamony High-Content Analysis Software

Usage fee (per hour): project dependent, please inquire.

Contact Person : Dr. Ursula Eilers, Dr. Christina Schülein-Völk

Room DK04, Core Facility Functional Genomics

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Leica DM6000B upright microscope - LMD6500 Laser Microdissection system

Objectives: 1.25x DRY, 5x Dry, 10x DRY, 20x DRY, 40x CORR, 40Lx CORR

(5x, 10x, 20x and 40Lx are suitable for laser microdissection)

Filter cubes: A4 (blue), L5 (green), Y5 (red)

Camera: DFC295 (digital color camera), DFC360FX (for fluorescence microscopy)

Usage fee (per hour): 20€ (internal Biocenter ), 35€ (external)

Contact Person: Beatrice Dankworth

Room B322, Core Facility Functional Genomics
 

Workstation for Image Analysis

Room DK08