AG Wolf
Contact
Prof. Dr. Elmar Wolf
Lehrstuhl für Biochemie und Molekularbiologie
Biozentrum
Am Hubland
97074 Würzburg
Raum B 311
Tel: 0931 31-83259
Fax: 0931 31-84113
elmar.wolf@biozentrum.uni-wuerzburg.de
Emmy Noether group - Cancer Systems Biology
Lab News
March 2023
Julia Wenzel joined our team for her master thesis - welcome, Julia!
January 2023
I am delighted and grateful to be awarded an ERC consolidator grant (#ERCCoG). In PROTAC-PDAC we will target oncogenic transcription factors in pancreatic cancer. I thank my research group, my colleagues in Würzburg and my many collaborators for their fantastic cooperation and help!
Our master students Marie and Katharina both successfully defended their work. Congratulations to both of you for a great thesis!
Our postdoc Mathias Diebold published his article about a covalently linked Aurora-A/MYCN complex. Congratulations, Mathias!
November 2022
Congratulations Daniel Solvie, Apoorva Baluapuri and Leonie Uhl for a fantastic publication in Nature about the multimerization of MYC!
Ashwin won the poster competition of the EUREKA! Symposium 2022 (organized by GSLS)! Congratulations!
October 2022
We have a new lab member - welcome Desiree!
September 2022
Congratulations to Jessica for publishing her work in Front Cell Dev Biol. together with Almut Schulze and team!
We have two new starts at the lab!!
Mathias Diebold joins us as a postdoc and Belinda continues with us for her master's thesis!
Archive
Research
In ageing Western societies tumors are becoming the most relevant medical problem. A key characteristic of tumors is deregulated signaling pathways that significantly alter the transcriptional profile of tumor cells. Proteins of the MYC family play a prominent role in this oncogenic reprogramming as transcription factors.
Our vision is to understand the transcriptional changes during carcinogenesis and, in particular, the oncogenic function of Myc to the extent that new pharmaceutical strategies for the fight against cancer can be developed on this basis. To this end, my research group uses genome- and proteome-wide methods to better understand the biology of tumors as well as genetic screens to find potential therapeutic targets of tumor cells. Together with our partners, we have recently developed substances that inhibit the function of Myc. In the next few years, we want to improve these molecules, of the PROTAC substance class, to such an extent that they can be clinically tested and applied.
Research topic 1: The oncogenic function of MYC
Research topic 2: Investigation of transcriptional elongation
Research topic 3: New pharmaceutical concepts in oncology
Publications
2021/2022
Daniel Solvie, Apoorva Baluapuri, Leonie Uhl, Daniel Fleischhauer, Theresa Endres, Dimitrios Papadopoulos, Amel Aziba, Abdallah Gaballa, Ivan Mikicic, Ekaterina Isaakova, Celeste Giansanti, Jennifer Jansen, Marvin Jungblut, Teresa Klein, Christina Schülein-Völk, Hans Maric, Sören Doose, Markus Sauer, Petra Beli, Andreas Rosenwald, Matthias Dobbelstein, Elmar Wolf*, Eilers* (2022) MYC multimers shield stalled replication forks from RNA polymerase. Nature doi: 10.1038/s41586-022-05469-4.
Otto C, Kastner C, Schmidt S, Uttinger K, Baluapuri A, Denk S, Rosenfeldt MT, Rosenwald A, Roehrig F, Ade CP, Schuelein-Voelk C, Diefenbacher ME, Germer CT, Wolf E, Eilers M, Wiegering A (2022) RNA polymerase I inhibition induces terminal differentiation, growth arrest and vulnerability to senolytics in colorectal cancer cells. Mol Oncol
Schwarz JD, Lukassen S, Bhandare P, Eing L, Snaebjörnsson MT, Cruz García Y, Kisker JP, Schulze A, Wolf E (2022)The glycolytic enzyme ALDOA and the exon junction complex protein RBM8A are regulators of ribosomal biogenesis. Frontiers in Cell and Developmental Biology doi: 10.3389/fcell.2022.954358
Bozilovic J, Eing L, Berger BT, Adhikari B, Weckesser J, Berner NB, Wilhelm S, Kuster B, Wolf E*, Knapp S* (2022) Novel, highly potent PROTACs targeting AURORA-A kinase. Current Research in Chemical Biology 2, IF: N/A
Doffo J, Bamopoulos SA, Köse H, Orben F, Zang C, Pons M, den Dekker AT, Brouwer RWW, Baluapuri A, Habringer S, Reichert M, Illendula A, Krämer OH, Schick M, Wolf E, van IJcken WFJ, Esposito I, Keller U, Schneider G, Wirth M. NOXA expression drives synthetic lethality to RUNX1 inhibition in pancreatic cancer. PNAS. 2022, 119(9).
Riedel A, Helal M, Pedro L, Swietlik JJ, Shorthouse D, Schmitz W, Haas L, Young T, da Costa ASH, Davidson S, Bhandare P, Wolf E, Hall BA, Frezza C, Oskarsson T, Shields JD. Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma. Cancer Immunol Res. 2022, 10(4):482-497
Schick M, Zhang L, Maurer S, Maurer HC, Isaakaidis K,Schneider L, Patra U, Schunck K, Rohleder E, Hofstetter J, Baluapuri A,Scherger AK, Slotta-Huspenina J, Hettler F, Weber J, Engleitner T, Maresch R,Slawska J, Lewis R, Istvanffy R, Habringer S, Steiger K, Baiker A, OostendorpRAJ, Miething C, Lenhof HP, Bassermann F, Chapuy B, Wirth M, Wolf E, Rad R, Müller S, Keller U (2022)Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis andgenetic instability in diffuse large B-cell lymphoma. Nature Communications 13
Narain A, Bhandare P, Adhikari B, Backes S, Eilers M, Dolken L, Schlosser A, Erhard* F, Baluapuri* A, Wolf* E (2021) Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination. Mol Cell 81: 3110-3127 e14 *corresponding authors
Dölle A, Adhikari B, Kramer A, Weckesser J, Berner N, Berger LM, Diebold M, Szewczyk MM, Barsyte-Lovejoy D, Arrowsmith CH, Gebel J, Lohr F, Dotsch V, Eilers M, Heinzlmeir S, Kuster B, Sotriffer C, Wolf* E, Knapp* S (2021) Design, Synthesis, and Evaluation of WD-Repeat-Containing Protein 5 (WDR5) Degraders. J Med Chem 64: 10682-10710 *corresponding authors
Adhikari* B, Narain A, Wolf* E (2021) Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cells. STAR Protoc 2: 100949 *corresponding authors
Papadopoulos D, Solvie D, Baluapuri A, Endres, T, Ha S., Herold S, Kalb J, Giansanti C, Schülein-Völk C, Ade CP, Schneider C, Gaballa A, Vos S, Fischer U, Dobbelstein M, Wolf E, Eilers M (2021) MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription‑replication conflicts. Mol Cell https://doi.org/10.1016/j.molcel.2021.11.002
Krenz B, Gebhardt-Wolf A, Ade CP, Gaballa A, Roehrig F, Vendelova E, Baluapuri A, Eilers U, Gallant P, D'Artista L, Wiegering A, Gasteiger G, Rosenfeldt MT, Bauer S, Zender L, Wolf E, Eilers M (2021) MYC- and MIZ1-Dependent Vesicular Transport of Double-Strand RNA Controls Immune Evasion in Pancreatic Ductal Adenocarcinoma. Cancer Res 81: 4242-4256
Endres T, Solvie D, Heidelberger JB, Andrioletti V, Baluapuri A, Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, Zuber J, Beli P, Popov N, Wolf E, Gallant P, Eilers M (2021) Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters. Mol Cell 81: 830-844 e13
Sipol A, Hameister E, Xue B, Hofstetter J, Barenboim M, Ollinger R, Jain G, Prexler C, Rubio RA, Baldauf MC, Franchina DG, Petry A, Schmah J, Thiel U, Gorlach A, Cario G, Brenner D, Richter G, Grunewald TGP, Rad R, Wolf E, Ruland J, Sorensen PH, Burdach SEG (2021) MondoA Drives B-ALL Malignancy through Enhanced Adaptation to Metabolic Stress. Blood
Wolpaw AJ, Bayliss R, Buchel G, Dang CV, Eilers M, Gustafson WC, Hansen GH, Jura N, Knapp S, Lemmon MA, Levens D, Maris JM, Marmorstein R, Metallo SJ, Park JR, Penn LZ, Rape M, Roussel MF, Shokat KM, Tansey WP, Verba KA, Vos SM, Weiss WA, Wolf E, Mosse YP (2021) Drugging the "Undruggable" MYCN Oncogenic Transcription Factor: Overcoming Previous Obstacles to Impact Childhood Cancers. Cancer Res 81: 1627-1632
Hartmann O, Reissland M, Maier CR, Fischer T, Prieto-Garcia C, Baluapuri A, Schwarz J, Schmitz W, Garrido-Rodriguez M, Pahor N, Davies CC, Bassermann F, Orian A, Wolf E, Schulze A, Calzado MA, Rosenfeldt MT, Diefenbacher ME (2021) Implementation of CRISPR/Cas9 Genome Editing to Generate Murine Lung Cancer Models That Depict the Mutational Landscape of Human Disease. Front Cell Dev Biol 9: 641618
