Lehrstuhl für Biotechnologie und Biophysik



    The rapidSTORM project is an open source evaluation tool that provides fast and highly configurable data processing for single-molecule localization microscopy such as dSTORM. It provides both two-dimensional and three-dimensional, multi-color data analysis as well as a wide range of filtering and image generation capabilities. The general operation of rapidSTORM is described in this article.

    rapidSTORM is currently available in two versions: rapidSTORM 2 is the current stable branch, where only outstanding bugs are fixed, while rapidSTORM 3 is the unstable development branch with an improved user interface and simplified technical deployment.

    News, updates and discussion

    You can track announcements, news and new releases for rapidSTORM by subscribing to the rapidstorm-announce mailing list. This list is very low volume (typically less than one mail per month) and read-only. Your questions, feedback, and discussions about rapidSTORM are very welcome on the rapidstorm-discuss list.

    rapidSTORM features
    Basic rapidSTORM features
    FeaturerapidSTORM 1rapidSTORM 2/3
    Graphical user interfacexx
    Command line interfacexx
    Easy scriptability and testability through simparm frameworkxx
    Reading Andor SIF filesxx
    Reading and writing TIFF filesxx
    Direct connection to Andor camerasxx
    Pseudorandom input data simulation with true Besselian PSFsxx
    Input simulation for 3D PSFs x
    Least-squares fittingxx
    Data analysis in secondsxx
    Modular, easily extensible architecturexx
    rapidSTORM processing features
    FeaturerapidSTORM 1rapidSTORM 2/3
    Least-squares fittingxx
    MLE fitting x
    2D processing with known PSFxx
    2D fitting with per-spot PSF widthxx
    Estimation of PSF from input dataxx
    Estimation of 3D PSF from input dataxx
    3D using astigmatism x
    3D using biplane x
    Multi-color and multi-plane support x
    Two-kernel analysis for multi-spot eventsxx
    rapidSTORM result analysis features
    FeaturerapidSTORM 1rapidSTORM 2/3
    Online result view during analysis xx
    High & adaptable image contrast through WHNxx
    Time-resolved imagesxx
    Can create time-lapse moviesxx
    Free selection of coordinate and hue axes in image generation x
    Online filtering of results during analysisxx
    Advanced result filtering using arithmetic expressions x
    Easy linear drift correctionxx
    Joining multiple time-consecutive localizations by Kalman trackingxx
    Download and installation

    Our Webserver was hit by a Webdefacemant, so meanwile have a look here.

    Documentation and support

    The rapidSTORM manual is available online in HTML format or PDF format.

    We are grateful for any comments and bug reports on the rapidSTORM software and strive for active development of the software. Please report your bugs and comments to the bug tracking system. The rapidSTORM framework is also highly extensible and an excellent starting position for developing and testing new algorithms for super-resolution microscopy; please contact Steve Wolter if you are interested in such collaboration.


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