The rapidSTORM project is an open source evaluation tool that provides fast and highly configurable data processing for single-molecule localization microscopy such as dSTORM. It provides both two-dimensional and three-dimensional, multi-color data analysis as well as a wide range of filtering and image generation capabilities. The general operation of rapidSTORM is described in this article.

rapidSTORM is currently available in two versions: rapidSTORM 2 is the current stable branch, where only outstanding bugs are fixed, while rapidSTORM 3 is the unstable development branch with an improved user interface and simplified technical deployment.

You can track announcements, news and new releases for rapidSTORM by subscribing to the rapidstorm-announce mailing list. This list is very low volume (typically less than one mail per month) and read-only. Your questions, feedback, and discussions about rapidSTORM are very welcome on the rapidstorm-discuss list.

Basic rapidSTORM features
Feature rapidSTORM 1 rapidSTORM 2/3
Graphical user interface x x
Command line interface x x
Easy scriptability and testability through simparm framework x x
Reading Andor SIF files x x
Reading and writing TIFF files x x
Direct connection to Andor cameras x x
Pseudorandom input data simulation with true Besselian PSFs x x
Input simulation for 3D PSFs   x
Least-squares fitting x x
Data analysis in seconds x x
Modular, easily extensible architecture x x
rapidSTORM processing features
Feature rapidSTORM 1 rapidSTORM 2/3
Least-squares fitting x x
MLE fitting   x
2D processing with known PSF x x
2D fitting with per-spot PSF width x x
Estimation of PSF from input data x x
Estimation of 3D PSF from input data x x
3D using astigmatism   x
3D using biplane   x
Multi-color and multi-plane support   x
Two-kernel analysis for multi-spot events x x
rapidSTORM result analysis features
Feature rapidSTORM 1 rapidSTORM 2/3
Online result view during analysis x x
High & adaptable image contrast through WHN x x
Time-resolved images x x
Can create time-lapse movies x x
Free selection of coordinate and hue axes in image generation   x
Online filtering of results during analysis x x
Advanced result filtering using arithmetic expressions   x
Easy linear drift correction x x
Joining multiple time-consecutive localizations by Kalman tracking x x

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