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    Lehrstuhl für Biotechnologie und Biophysik

    Re-scan Confocal Microscopy (RCM)

    We set up a correlative system using the super-resolution technique dSTORM and Re-scan Confocal Microscopy (RCM), which is a resolution-enhanced form of LSM. By re-scanning the emission light after the pinhole, RCM gains a resolution improvement of 1.4 fold. This will increase the lateral resolution from 240 nm of a confocal microscope to 170 nm of the RCM, while maintaining the sectioning capability of a confocal system. Since the detector (Photomultiplier Tube) is replaced by a sCMOS camera with high quantum efficiency, the signal-to-noise ratio of the RCM is twice as good as for a confocal microscope. With this system we are now capable of quantitatively investigating protein distributions in the context of dynamic processes with high spatial resolution.

    Reference: de Luca et al., 2013

    Microscope-body: Nikon TiE with motorized stage and PFS-System (Perfect-Focus-System), inverted
    Objective: 100x Oil, NA 1.49 Apo-TIRF
    60x Water, NA 1.27
    100x Siliconoil, 1.3
    dSTORM Detection
    Laser: 405nm, 488nm, 640nm: 170mW, 532nm: 500mW
    Camera:EMCCD, Andor iXON DU-897
    RCM
    Laser: Cobolt Skyra, Multiline lasersystem
    405nm, 488nm, 561nm, 640nm: 50mW
    Camera:sCMOS, Andor Zyla 4.2P
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    Lehrstuhl für Biotechnologie und Biophysik
    Am Hubland
    97074 Würzburg

    Tel.: +49 931 31-84507
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