Structured Illumination Microscopy (SIM)

Reference: Gustafsson et al., 2008

Zeiss Elyra S.1 SIM

Microscope-body: Zeiss Elyra S.1 SIM
Objective: 10x dry, 63x oil
Laser: 405 nm, 488 nm, 561 nm, 642 nm
Cameras: sCMOS, PCO Edge 5.5
Part of the Imaging Core Facility (Room CK01)
Usage fee (per hour):20€ (internal BIOC.) 35€ (external)
Contact Person:Marcus Behringer
Live cell 3 colour SIM of COS7 cells stained targets: - nucleus: Hoechst 33342 - tubulin: Tubulin Tracker green - plasma membrane: CellMask orange
Live cell 3 colour SIM of COS7 cells stained targets: - nucleus: Hoechst 33342 - tubulin: Tubulin Tracker green - plasma membrane: CellMask orange
Live cell 4 colour SIM of U2-OS cells stained targets: - nucleus: Hoechst 33342 - tubulin: Tubulin Tracker green - mitochondria: MitoTracker orange - plasma membrane: CellMask deep red