"Dass ich nicht mehr mit sauerm Schweiß Zu sagen brauche, was ich nicht weiß; Dass ich erkenne, was die Welt Im Innersten zusammenhält." Johann Wolfgang von Goethe, Faust
... is a molecular biologist with research experience in academia and industry. He completed his PhD at the University of Oxford where he developed novel CRISPR screening and gene editing approaches (LeishGEdit) to study the requirement of parasite motility throughout the Leishmania life cycle. This was followed by a PostDoc at the University of Oxford and a research position in the biotech industry. At OXGENE he employed large-scale CRISPR screens for target discovery projects. Tom has now been awarded an EMBO and Marie Curie Fellowship to continue his research on Leishmania at the University of Würzburg and envisions to set up his own group at the Biocenter.
Since 2022: EMBO and Marie Curie Fellowship in Markus Engstler’s lab, Cell and Developmental Biology, Biocenter, University of Würzburg
2020 – 2022: CRISPR Screening Scientist/Senior Scientist, Oxford Genetics (OXGENE)
2019 – 2020: PostDoc in William James’ lab, Sir William Dunn School of Pathology, University of Oxford
2015 – 2019: PhD Student in Eva Gluenz’ lab, Sir William Dunn School of Pathology, University of Oxford
Tom is studying the protozoan parasite Leishmania which causes the neglected tropical disease leishmaniasis. This devastating disease causes approximately one million new cases and at least 20,000 deaths around the globe every year (WHO, 2019). Leishmania species are transmitted into humans by the bite of a sand fly. Once inside their mammalian host, they predominantly enter macrophages and can remain at the site of the bite (cutaneous leishmaniasis), disseminate throughout the skin (diffuse cutaneous/mucocutaneous leishmaniasis), or enter the bloodstream to establish visceral infections (visceral leishmaniasis). This is the difference between a mild illness or a deadly disease, and the fundamental mechanisms behind these different clinical manifestations are not fully understood.
Tom’s research aims to dissect these mechanisms. In particular, he wants to test the hypothesis that different Leishmania species alter macrophage migration modes in distinct ways, to control dissemination through the dermis and across endothelial barriers. To determine the dynamics of parasite dissemination, he plans to use pipelines and methods that have been developed in the Engstler lab, including specialised microscopy techniques, high-throughput microfluidics and advanced 3D tissue models. In addition, Tom is planning to set up high-throughput CRISPR screens in both, parasites and macrophages, to functionally dissect species-dependent mechanisms that enable Leishmania parasites to disseminate in their mammalian host. To pursue functional genetic screens in Leishmania, Tom has recently developed a new method (LeishBASEedit) which enables for the first time loss-of-function screens in Leishmania via delivery of plasmid libraries.
Beneke T., Dobramysl U., Catta-Preta C. M. C., Mottram C. J., Gluenz E., Wheeler R. (2022). Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase. Wellcome Open Research, 7. (Link)
Sharma R., Avendaño Rangel F., Reis-Cunha J. L., Marques L. P., Figueira C. P., Borba P. B., Viana S. M., Beneke T., Bartholomeu D. C. and de Oliveira C. I. (2022). Targeted Deletion of Centrin in Leishmania braziliensis Using CRISPR-Cas9-Based Editing. Frontiers in Cellular and Infection Microbiology, 11. (Link)
Espada C. R., Quilles J. C., Albuquerque-Wendt A., Cruz M. C., Beneke T., Lorenzon L. B., Gluenz E., Cruz A. K. and Uliana S. R. B. (2021). Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase. Frontiers in Cellular and Infection Microbiology, 11. (Link)
Andersson M., [...], Beneke T. et al. (2020). SARS-CoV-2 RNA detected in blood samples from patients with COVID-19 is not associated with infectious virus. Wellcome Open Research Oct 12;5:181. (authors in alphabetically order). (Link)
Wang Z.*, Beneke T.*, Gluenz E. and Wheeler R. J. (2020). The single flagellum of Leishmania has a fixed polarisation of its asymmetric beat. Journal of Cell Science 133: jcs246637. (Link)
Beneke T., Banecki K., Fochler S. and Gluenz E. (2020). LAX28 is required for assembly of the inner dynein arm l1 and tether/tether head complex in the Leishmania flagellum. Journal of Cell Science 133: jcs239855. (Link)
Beneke T. and Gluenz E. (2020). Bar-seq strategies for the LeishGEdit toolbox. Molecular and Biochemical Parasitology 239, 111295. (Link)
Beneke T., Demay F., Hookway E., Ashman N., Jeffery H., Smith J., Valli J., Becvar T., Myskova J., Lestinova T., Shafiq S., Sadlova J., Volf P., Wheeler R. J., and Gluenz E. (2019). Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections. PLoS Pathog 15, e1007828. (Link)
Schädeli D., Serricchio M., Ben Hamidane H., Loffreda A., Hemphill A., Beneke T., Gluenz E., Graumann J. and Bütikofer P. (2019). Cardiolipin depletion-induced changes in the Trypanosoma brucei proteome. FASEB J Dec;33(12):13161-13175. (Link)
Costa F. C., Francisco A. F., Jayawardhana S., Calderano S. G., Lewis M. D., Olmo F., Beneke T., Gluenz E., Sunter J., Dean S., Kelly J. M. and Taylor M. (2018). Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping. PLoS Neg Trop Dis 12, e0006388. (Link)
Martel D., Beneke T., Gluenz E., Spaeth G. and Rachidi N. (2017). Characterisation of Casein kinase 1.1 in Leishmania donovani using the CRISPR Cas 9 toolkit. BioMed Res International 2017:4635605. (Link)
Beneke T.*, Madden R.*, Makin L., Valli J., Sunter J. and Gluenz E. (2017). A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. R Soc Open Sci 4, 170095. (Link)
Beneke T., Engstler M. (2022). Introducing the CRISPR/Cas9 cytosine base editor toolbox ‘LeishBASEedit’ – Gene editing and high-throughput screening in Leishmania without requiring DNA double-strand breaks, homologous recombination or donor DNA. BioRxiv. (Link)
McCoy C. J., Paupelin-Vaucelle H., Gorilak P., Beneke T., Varga V. and Gluenz E. (2022). ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum. BioRxiv. (Link)
Beneke T., Demay F., Wheeler R. J. and Gluenz E. (2020). Isolation of Leishmania promastigote flagella. Methods in Molecular Biology Trypanosomatids 2116:485-495. (Link)
Beneke T. and Gluenz E. (2019). LeishGEdit: a method for rapid gene knockout and tagging using CRISPR/Cas9. Methods in Molecular Biology Trypanosomatids 1971:189-210. (Link)