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Lehrstuhl für Zell- und Entwicklungsbiologie

Markus Engstler

Corpora non agunt nisi fixata ...

Lehrstuhl für Zell- und Entwicklungsbiologie

Theodor-Boveri-Institut
Biozentrum der Universität Würzburg
Am Hubland
97074 Würzburg

Fon & Fax

0049 931 3184250 (Uli Borst & Manu Engert)
0049 931 3180060 (Markus Engstler)
0049 931 3184252 (Fax)

Mail

zeb(at)uni-wuerzburg.de (Uli Borst & Manu Engert)
markus.engstler(at)uni-wuerzburg.de

Markus Engstler

... is a molecular cell biologist with substantial interest in infection biology and (bio)physics. He graduated from Christian Albrecht University of Kiel where he also received a doctorate in biochemistry. Markus spent his postdoctoral time at The Rockefeller University (New York) and the Max Planck Institute of Biochemistry (Martinsried). He further developed his quantitative approaches to study cells as a project group leader at the Free University of Berlin and the Ludwig Maximilian University of Munich. Following habilitation in Genetics (LMU Munich), he was appointed Professor of Genetics at Darmstadt University of Technology. Since 2009, Markus Engstler is Professor and Chair of the Department of Cell and Developmental Biology at the Julius-Maximilians-University of Würzburg. Among other duties, he continues to act as a member of numerous scientific advisory boards, as well as an editor for several journals. He has served as dean and vice-dean of faculty, and is founding director of the Center for Computational and Theoretical Biology (CCTB). Markus Engstler is also a founding member of DNTDs, the German Network Against Neglected Tropical Diseases. He is acting president of the German Society for Parasitology (DGP) and serves on the board of directors of the German Life Sciences Association (VBIO.) Recently he has initiated and co-founded the German Center for Multisectorial Fight against Neglected Tropical Diseases (DZVT). Since 2021, Markus Engstler is speaker of the new DFG priority programme SPP 2332 "Physics of Parasitism" 

since 2009 Chair and Professor of Cell and Developmental Biology, University of Würzburg

2006 - 2009 Professor of Genetics, Technical 
University, Darmstadt

2004 Habilitation, LMU Munich

2001-2004 Research Project Leader, LMU 
Munich

1998-2001 Senior Staff Scientist, Free University of Berlin

1996-1998 Postdoc, MPI Martinsried

1994-1996 Postdoc, The Rockefeller University, New York

1994-1999 DKFZ postdoctoral fellow

1994 Dr. rer. nat, University of Kiel

Research synopsis

We have been using African trypanosomes as a cell biology model for 30 years. Early biochemical work unraveled trans-sialylation, a novel mechanism of protein glycosylation, which was later shown to be critically involved in pathogenesis. Research on the control of parasite development led to new paradigms for the molecular basis of trypanosome stage differentiation. Cold-shock and chemical sensing function cooperatively in the initiation of stage transition and the routing of the major surface coat proteins is differentially regulated throughout the parasite’s life cycle. The characterization and mapping of the endocytic recycling pathways in T. brucei using quantitative fluorescence and EM methods has paved the way for trypanosomes to become a more generally accepted model system. The kinetics of plasma membrane recycling is extremely fast and the parasites harbour morphologically and functionally well-defined endosomes. A novel mechanism for the sorting of GPI-anchored proteins appears to be present (not only) in trypanosomes. This work also suggested that rapid endocytosis could be essential for trypanosome survival. The parasites continuously swim and thereby generate directional flow fields on their cell surface. These flow forces become functional when the surface coat, which is dominated by variant surface glycoproteins (VSG), is attacked by host immunoglobulins. Hydrodynamic forces drag antibody-VSG complexes towards the rear of the cell, where they are endocytosed. Thus, pure physical forces can sort proteins in the plane of the plasma membrane.

Antibody clearance raised the question how trypanosomes actually swim and if this phenomenon is functional in natural infection. This could only be addressed in collaboration with partners in Africa on the one hand, and physicists and mathematicians on the other hand. Trypanosomes reveal an amazingly complex motion behaviour in vitro and in the diverse and crowded environments of the host. Different trypanosome species have adapted to distinct infection niches, such as the circulation or tissue spaces. And the parasites not only swim in the mammal, but also in the insect vector, where they form giant swarms.

Most recent work unraveled that trypanosomes are dynamic at various scales: the first complete structures of VSGs revealed an unprecedented molecular flexibility that accounts for different populations of VSGs on the cell surface of trypanosomes diffusing at different rates. Overcrowding of the VSG coat leads to rapid formation of nanotubes and nanovesicles that - by purely physical forces - are shed form the cell surface.

Thus, part our work will continue focussing on the mechanical forces acting within, on and around different trypanosome species. The latest idea that we plan to test is that mechanical adaptations allow avoidance of interspecies competition in the same host. This study is  Project 4  of the SPP 2332 PoP.

 

 

Recent Publications

Response to comment on 'Unexpected plasticity in the life cycle of Trypanosoma Brucei'.
Lisack J, Morriswood B, Engstler M.
Elife. 2022 Feb 1;11:e75922. doi: 10.7554/eLife.75922. PMID: 35103593

The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids.
Borges AR, Link F, Engstler M, Jones NG.
Front Cell Dev Biol. 2021 Nov 1;9:720536. doi: 10.3389/fcell.2021.720536. eCollection 2021. PMID: 34790656

Nanobody-mediated macromolecular crowding induces membrane fission and remodeling in the African trypanosome.
Hempelmann A, Hartleb L, van Straaten M, Hashemi H, Zeelen JP, Bongers K, Papavasiliou FN, Engstler M, Stebbins CE, Jones NG.
Cell Rep. 2021 Nov 2;37(5):109923. doi: 10.1016/j.celrep.2021.109923. PMID: 34731611

The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei.
Bakari-Soale M, Ikenga NJ, Scheibe M, Butter F, Jones NG, Kramer S, Engstler M.
Sci Rep. 2021 Sep 15;11(1):18325. doi: 10.1038/s41598-021-97020-0. PMID: 34526538

To the Surface and Back: Exo- and Endocytic Pathways in Trypanosoma brucei.
Link F, Borges AR, Jones NG, Engstler M.
Front Cell Dev Biol. 2021 Aug 6;9:720521. doi: 10.3389/fcell.2021.720521. eCollection 2021. PMID: 34422837

Unexpected plasticity in the life cycle of Trypanosoma brucei.
Schuster S, Lisack J, Subota I, Zimmermann H, Reuter C, Mueller T, Morriswood B, Engstler M.
Elife. 2021 Aug 6;10:e66028. doi: 10.7554/eLife.66028. PMID: 34355698

Single-cell motile behaviour of [Formula: see text] in thin-layered fluid collectives.
Krüger T, Maus K, Kreß V, Meyer-Natus E, Engstler M.
Eur Phys J E Soft Matter. 2021 Mar 23;44(3):37. doi: 10.1140/epje/s10189-021-00052-7. PMID: 33755816

Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples.
Kramer S, Meyer-Natus E, Stigloher C, Thoma H, Schnaufer A, Engstler M.
Nucleic Acids Res. 2021 Feb 22;49(3):e14. doi: 10.1093/nar/gkaa1142. PMID: 33275141

Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus.
Rose C, Casas-Sánchez A, Dyer NA, Solórzano C, Beckett AJ, Middlehurst B, Marcello M, Haines LR, Lisack J, Engstler M, Lehane MJ, Prior IA, Acosta-Serrano Á.
Nat Microbiol. 2020 Jul;5(7):909-916. doi: 10.1038/s41564-020-0707-z. Epub 2020 Apr 20. PMID: 32313202